IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049, Madrid, Spain; Departamento de Química Analítica y Análisis Instrumental. Universidad Autónoma de Madrid, 28049, Madrid, Spain.
Departamento de Química Analítica y Análisis Instrumental. Universidad Autónoma de Madrid, 28049, Madrid, Spain.
Biosens Bioelectron. 2024 Oct 1;261:116500. doi: 10.1016/j.bios.2024.116500. Epub 2024 Jun 13.
In this work, we present an electrochemical sensor for fast, low-cost, and easy detection of the SARS-CoV-2 spike protein in infected patients. The sensor is based on a selected combination of nanomaterials with a specific purpose. A bioconjugate formed by Few-layer bismuthene nanosheets (FLB) and tetrahedral DNA nanostructures (TDNs) is immobilized on Carbon Screen-Printed Electrodes (CSPE). The TDNs contain on the top vertex an aptamer that specifically binds to the SARS-CoV-2 spike protein, and a thiol group at the three basal vertices to anchor to the FLB. The TDNs are also marked with a redox indicator, Azure A (AA), which allows the direct detection of SARS-CoV-2 spike protein through changes in the current intensity of its electrolysis before and after the biorecognition reaction. The developed sensor can detect SARS-CoV-2 spike protein with a detection limit of 1.74 fg mL directly in nasopharyngeal swab human samples. Therefore, this study offers a new strategy for rapid virus detection since it is versatile enough for different viruses and pathogens.
在这项工作中，我们提出了一种电化学传感器，用于快速、低成本、轻松地检测感染患者中的 SARS-CoV-2 刺突蛋白。该传感器基于具有特定用途的纳米材料的精选组合。由少层二碲化铋纳米片 (FLB) 和四面体形 DNA 纳米结构 (TDN) 形成的生物缀合物固定在碳丝网印刷电极 (CSPE) 上。TDN 在顶部顶点包含与 SARS-CoV-2 刺突蛋白特异性结合的适体，在三个底顶点包含巯基以锚定到 FLB。TDN 还标记有氧化还原指示剂 Azure A (AA)，这允许通过在生物识别反应前后其电解的电流强度的变化直接检测 SARS-CoV-2 刺突蛋白。开发的传感器可以在鼻咽拭子人样本中直接检测到检测限为 1.74 fg mL 的 SARS-CoV-2 刺突蛋白。因此，由于它对不同的病毒和病原体具有足够的通用性，因此该研究为快速病毒检测提供了一种新策略。
