Department of Chemistry, Faculty of Sciences, Ilam University, Ilam, P.O. BOX. 69315-516, Iran.
Department of Chemistry, Faculty of Sciences, Ilam University, Ilam, P.O. BOX. 69315-516, Iran.
Bioelectrochemistry. 2022 Aug;146:108106. doi: 10.1016/j.bioelechem.2022.108106. Epub 2022 Mar 23.
The development of advanced electrode materials and the combination of aptamer with them have improved dramatically the performance of aptasensors. Herein, a new architecture based on copper hydroxide nanorods (Cu(OH) NRs) are directly grown on the surface of screen printed carbon electrode (SPCE) using a two-step in situ, very simple and fast strategy and was used as a high-performance substrate for immobilization of aptamer strings, as well as an electrochemical probe to development a label-free electrochemical aptasensor for SARS-CoV-2 spike glycoprotein measurement. The Cu(OH) NRs was characterized using X-ray Diffraction (XRD) and electron microscopy (FESEM). In the presence of SARS-CoV-2 spike glycoprotein, a decrease in Cu(OH) NRs-associated peak current was observed that can be owing to the target-aptamer complexes formation and thus blocking the electron transfer of Cu(OH) NRs on the surface of electrode. This strategy exhibited wide dynamic range in of 0.1 fg mL to 1.2 µg mL and with a high sensitivity of 1974.43 μA mM cm and low detection limit of 0.03 ± 0.01 fg mL of SARS-CoV-2 spike glycoprotein deprived of any cross-reactivity in the presence of possible interference species. In addition, the good reproducibility, repeatability, high stability and excellent feasibility in real samples of saliva and viral transport medium (VTM) were found from the provided aptasensor. Also, the aptasensor efficiency was evaluated by real samples of sick and healthy individuals and compared with the standard polymerase chain reaction (PCR) method and acceptable results were observed.
先进电极材料的发展以及适配体与它们的结合，极大地提高了适配体传感器的性能。在此，通过两步原位、非常简单和快速的策略，在丝网印刷碳电极（SPCE）表面直接生长氧化铜纳米棒（Cu（OH）NRs），并将其用作固定适配体链的高性能基底，以及电化学探针，开发用于 SARS-CoV-2 刺突糖蛋白测量的无标记电化学适配体传感器。Cu（OH）NRs 采用 X 射线衍射（XRD）和电子显微镜（FESEM）进行了表征。在 SARS-CoV-2 刺突糖蛋白存在的情况下，观察到与 Cu（OH）NRs 相关的峰电流减小，这可能是由于目标-适配体复合物的形成，从而阻止了 Cu（OH）NRs 在电极表面的电子转移。该策略在 0.1 fg mL 至 1.2 µg mL 的宽动态范围内表现出高灵敏度为 1974.43 μA mM cm 和低检测限为 0.03 ± 0.01 fg mL 的 SARS-CoV-2 刺突糖蛋白，且在存在可能的干扰物质的情况下无交叉反应。此外，从提供的适配体传感器中发现了良好的重现性、重复性、高稳定性和在唾液和病毒运输介质（VTM）实际样品中的出色可行性。此外，通过对患病和健康个体的实际样品评估了适配体传感器的效率，并与标准聚合酶链反应（PCR）方法进行了比较，观察到了可以接受的结果。
