Key Laboratory of Chemo/Biosensing and Detection, Xuchang University, Xuchang 461000, PR China; College of Chemical and Materials Engineering, Xuchang University, Xuchang 461000, PR China.
Key Laboratory of Chemo/Biosensing and Detection, Xuchang University, Xuchang 461000, PR China; College of Chemical and Materials Engineering, Xuchang University, Xuchang 461000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Nov 5;320:124665. doi: 10.1016/j.saa.2024.124665. Epub 2024 Jun 15.
As one of endogenous reactive oxygen species (ROS), peroxynitrite (ONOO) performs various functions in both pathological and physiological mechanisms. In this work, an optical and near-infrared (NIR) fluorescent probe (NX), which based on 3-dihydro-1H-xanthene and 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) group was designed and prepared to detect ONOO. This probe revealed an obvious optical and a fluorescent response when ONOO was present and it exhibited higher selectivity over other ROS. Especially, the dual NIR fluorescence changes at 660 and 800 nm allowed quantitative detection of ONOO in the range of 15-40 μM, and the detection limit was 82 nM. Finally, the probe was effectively employed to visualize exogenous and endogenous ONOO in HepG2 cells and zebrafish, respectively. All the results indicated the dual NIR-channel probe could serve as a potent detecting tools in studying ONOO in vitro and in vivo.
作为内源性活性氧（ROS）之一，过氧亚硝酸盐（ONOO）在病理和生理机制中发挥着多种功能。在这项工作中，我们设计并制备了一种基于 3-二氢-1H-呫吨和 2-二氰基乙烯基-3-氰基-4,5,5-三甲基-2,5-二氢呋喃（TCF）基团的光学和近红外（NIR）荧光探针（NX）来检测 ONOO。当存在 ONOO 时，该探针表现出明显的光学和荧光响应，并且对其他 ROS 具有更高的选择性。特别是，660nm 和 800nm 处的双近红外荧光变化允许在 15-40μM 的范围内对 ONOO 进行定量检测，检测限为 82nM。最后，该探针分别有效地用于在 HepG2 细胞和斑马鱼中可视化外源性和内源性 ONOO。所有结果表明，双近红外通道探针可以作为研究体外和体内 ONOO 的有力检测工具。
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