Dental Research Center, Dentistry Research Institute, Department of Restorative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Department of Restorative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Photodiagnosis Photodyn Ther. 2024 Aug;48:104249. doi: 10.1016/j.pdpdt.2024.104249. Epub 2024 Jun 17.
This study aimed to evaluate the impact of photodynamic therapy (PDT) using two photosensitizers, methylene blue and indocyanine green, on the microtensile bond strength of a universal adhesive to caries-affected dentin.
The occlusal enamel of 60 third molars was cut to expose the inner one-third of the dentin. Artificial caries were induced through a pH cycling process. The samples were divided into three groups: M (Methylene blue+ diode laser), I (Indocyanine green agents+ diode laser irradiation), and control. Each group was further divided into two subgroups according to the adhesive protocol (self-etch, total-etch). After restoring with Gradia composite resin, teeth were sectioned and exposed to 5000 thermal cycles. Microtensile bond strength was measured using a universal testing machine. The data were subjected to two- and one-way ANOVA and paired comparisons were performed by the Tamhane and Tukey tests.
The study found significant effects of the photosensitizer, etching pattern, and their interactions on the microtensile bond strength of composite resin to caries-affected dentin (P < 0.001). In the self-etching mode, PDT with indocyanine green exhibited significantly higher bond strength values compared to PDT with methylene blue (P = 0.001) and the control groups (P < 0.001). However, no significant differences were observed in the total-etch mode. (P = 0.54).
The etching mode played a more significant role in the bond strength when using the universal adhesive alongside PDT with methylene blue and indocyanine green. Employing two photosensitizers in PDT during the self-etch mode significantly increased the bond strength values.
本研究旨在评估两种光敏剂（亚甲蓝和吲哚菁绿）的光动力疗法（PDT）对龋损牙本质通用粘结剂的微拉伸粘结强度的影响。
将 60 颗第三磨牙的咬合釉质切割，暴露牙本质的内三分之一。通过 pH 循环过程诱导人工龋。将样本分为三组：M（亚甲蓝+二极管激光）、I（吲哚菁绿试剂+二极管激光照射）和对照组。根据粘结剂方案（自酸蚀、全酸蚀），每组进一步分为两个亚组。用 Gradia 复合树脂修复后，牙齿被切割并暴露于 5000 个热循环中。使用万能试验机测量微拉伸粘结强度。数据进行双因素和单因素方差分析，并用 Tamhane 和 Tukey 检验进行配对比较。
研究发现光敏剂、蚀刻模式及其相互作用对复合树脂与龋损牙本质的微拉伸粘结强度有显著影响（P < 0.001）。在自酸蚀模式下，与亚甲蓝 PDT 相比，吲哚菁绿 PDT 显示出更高的粘结强度值（P = 0.001）和对照组（P < 0.001）。然而，在全酸蚀模式下，没有观察到显著差异（P = 0.54）。
在使用通用粘结剂和亚甲蓝及吲哚菁绿的光动力疗法时，蚀刻模式对粘结强度的影响更大。在自酸蚀模式下，使用两种光敏剂的光动力疗法可显著提高粘结强度值。
