Department of Microbiology, Graduate School of Human Life and Ecology, Osaka Metropolitan University.
Osaka International Research Center for Infectious Diseases, Osaka Metropolitan University.
Microbes Environ. 2024;39(2). doi: 10.1264/jsme2.ME24011.
To investigate mycobacterial cases of farmed yellowtail fish in coastal areas of western Japan (Kagoshima, Kyushu), where aquaculture fisheries are active, Mycobacterium pseudoshottsii, the causative agent, was isolated from six neighboring fishing ports in 2012 and 2013. A phylogenetic ana-lysis revealed that the strains isolated from one fishing port were closely related to those isolated from other regions of Japan, suggesting the nationwide spread of a single strain. However, strains from Japan were phylogenetically distinct from those from the Mediterranean and the United States; therefore, worldwide transmission was not observed based on the limited data obtained on the strains exami-ned in this study. The present results demonstrate that a bacterial genomic ana-lysis of infected cases, a mole-cular epidemiology strategy for public health, provides useful data for estimating the prevalence and transmission pathways of M. pseudoshottsii in farmed fish. A bacterial genome ana-lysis of strains, such as that performed herein, may play an important role in monitoring the prevalence of this pathogen in fish farms and possible epidemics in the future as a result of international traffic, logistics, and trade in fisheries.
为了调查日本西部沿海地区（九州的鹿儿岛）养殖黄尾鱼的分枝杆菌病例，这些地区水产养殖业活跃，从 2012 年和 2013 年的六个相邻渔港分离出分枝杆菌假结核分枝杆菌，这种病原体是病因。系统发育分析表明，从一个渔港分离出的菌株与从日本其他地区分离出的菌株密切相关，表明单一菌株在全国范围内的传播。然而，日本的菌株与来自地中海和美国的菌株在系统发育上是不同的；因此，根据本研究中检查的菌株获得的有限数据，并未观察到全球传播。目前的结果表明，对感染病例进行细菌基因组分析，即公共卫生的分子流行病学策略，为评估养殖鱼类中分枝杆菌假结核分枝杆菌的流行情况和传播途径提供了有用的数据。对菌株进行细菌基因组分析，如本文所述，可能在监测鱼类养殖场中这种病原体的流行情况以及未来由于国际交通、物流和渔业贸易而可能发生的疫情方面发挥重要作用。
