Department of Microbiology, D.A.V. College, Lalitpur, Nepal.
BMC Res Notes. 2024 Jun 19;17(1):169. doi: 10.1186/s13104-024-06834-4.
The purpose of this study was to evaluate antibacterial activity of pigment extracted from bacteria, isolated from soil samples. During the study, 20 soil samples were collected from different areas (forest, agriculture fields, river sides and dumping sites) of Kathmandu and Lalitpur districts which were processed for isolation of pigment producing bacteria by spread plate technique. The pigmented bacterial isolates were identified and enriched in nutrient broth. Then, pigment was extracted in 95% methanol as solvent, which was further characterized using UV-Vis Spectrophotometric and TLC analysis. The obtained crude pigment extract was processed to carry out the antimicrobial susceptibility assay using agar well diffusion method. Out of 13 total pigmented bacteria isolates, four different colored pigmented bacterial isolates (S4O, S11Y, S14P and S17G) which produced efficient pigment on nutrient agar were chosen and they were further processed. Among these isolates, S4O was identified as Staphylococcus aureus, S11Y was identified as Micrococcus luteus, S14P was identified as Micrococcus roseus and S17G was identified as Pseudomonas aeruginosa respectively. On characterization using UV-Vis Spectrophotometric and TLC analysis, the pigment extracted from isolates S4O, S11Y and S14P were found to be Carotenoids and from isolate S17G was found to be Pyocyanin in nature. The maximum antibacterial activity was shown against Staphylococcus aureus from all the four pigments extracts. The green color pigment extract from isolate S17G was found to be most effective against all the Gram-positive and Gram-negative test bacteria. This study suggests that these pigment extracts from pigmented bacteria may have beneficial antibacterial roles that can be exploited in controlling unwanted bacterial growth.
本研究旨在评估从土壤样本中分离出的细菌提取的色素的抗菌活性。在研究过程中，从加德满都和勒利德布尔地区的不同区域（森林、农田、河边和倾倒场）采集了 20 个土壤样本，并通过平板划线技术对产色素细菌进行分离。将色素产生菌进行鉴定和富集于营养肉汤中，然后用 95%甲醇作为溶剂提取色素，进一步用 UV-Vis 分光光度法和 TLC 分析进行鉴定。获得的粗色素提取物用于采用琼脂孔扩散法进行抗菌药敏试验。在 13 个总产色素细菌分离株中，选择了 4 个不同颜色的产色素细菌分离株（S4O、S11Y、S14P 和 S17G），它们在营养琼脂上产生有效的色素，并进一步进行处理。在这些分离株中，S4O 被鉴定为金黄色葡萄球菌，S11Y 被鉴定为微球菌，S14P 被鉴定为玫瑰色微球菌，S17G 被鉴定为铜绿假单胞菌。通过 UV-Vis 分光光度法和 TLC 分析的鉴定，从分离株 S4O、S11Y 和 S14P 中提取的色素被发现为类胡萝卜素，而从分离株 S17G 中提取的色素为绿脓菌素。从所有四种色素提取物中，对金黄色葡萄球菌的抗菌活性最大。从分离株 S17G 中提取的绿色色素提取物对所有革兰氏阳性和革兰氏阴性测试菌均表现出最有效的抑菌活性。本研究表明，这些产色素细菌的色素提取物可能具有有益的抗菌作用，可以用于控制不需要的细菌生长。
