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通过在两系杂交稻中聚合新型 OsNramp5 和 OsLsi2 等位基因，生产超低重金属积累的稻谷。

Production of grains with ultra-low heavy metal accumulation by pyramiding novel Alleles of OsNramp5 and OsLsi2 in two-line hybrid rice.

机构信息

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, People's Republic of China.

出版信息

Plant Biotechnol J. 2024 Oct;22(10):2921-2931. doi: 10.1111/pbi.14414. Epub 2024 Jun 19.

DOI:10.1111/pbi.14414
PMID:38898780
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11536454/
Abstract

Ensuring rice yield and grain safety quality are vital for human health. In this study, we developed two-line hybrid rice (TLHR) with ultra-low grain cadmium (Cd) and arsenic (As) accumulation by pyramiding novel alleles of OsNramp5 and OsLsi2. We first generated low Cd accumulation restorer (R) lines by editing OsNramp5, OsLCD, and OsLCT1 in japonica and indica. After confirming that OsNramp5 was most efficient in reducing Cd, we edited this gene in C815S, a genic male sterile line (GMSL), and screened it for alleles with low Cd accumulation. Next, we generated R and GMSL lines with low As accumulation by editing OsLsi2 in a series of YK17 and C815S lines. When cultivated in soils that were heavily polluted with Cd and As, the edited R, GMSL, and TLHR plants showed significantly reduced heavy metal accumulation, while maintaining a relatively stable yield potential. This study provides an effective scheme for the safe production of grains in As- and/or Cd-polluted paddy fields.

摘要

确保水稻的产量和粮食安全质量对人类健康至关重要。在本研究中，我们通过聚合新型 OsNramp5 和 OsLsi2 等位基因，培育出具有超低谷物镉（Cd）和砷（As）积累的两系杂交稻（TLHR）。我们首先通过编辑粳稻和籼稻中的 OsNramp5、OsLCD 和 OsLCT1 来生成低 Cd 积累恢复系（R）。在确认 OsNramp5 在降低 Cd 方面最有效后，我们编辑了 C815S（一种基因雄性不育系（GMSL）中的该基因，并对具有低 Cd 积累的等位基因进行了筛选。接下来，我们通过编辑一系列 YK17 和 C815S 系中的 OsLsi2，生成了低 As 积累的 R 和 GMSL 系。当在 Cd 和 As 污染严重的土壤中种植编辑后的 R、GMSL 和 TLHR 植株时，重金属积累明显减少，而产量潜力保持相对稳定。本研究为在 As 和/或 Cd 污染的稻田中安全生产粮食提供了有效的方案。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/941851cab531/PBI-22-2921-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/1d06324fcbc5/PBI-22-2921-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/12a27908bb06/PBI-22-2921-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/464b20e18fc0/PBI-22-2921-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/964e290c3b5f/PBI-22-2921-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/34368cc36912/PBI-22-2921-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/941851cab531/PBI-22-2921-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/1d06324fcbc5/PBI-22-2921-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/12a27908bb06/PBI-22-2921-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/464b20e18fc0/PBI-22-2921-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/964e290c3b5f/PBI-22-2921-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/34368cc36912/PBI-22-2921-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8c4/11536454/941851cab531/PBI-22-2921-g002.jpg

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Stress Biol. 2023 Aug 21;3(1):34. doi: 10.1007/s44154-023-00117-x.
2
A combined strategy to mitigate the accumulation of arsenic and cadmium in rice (Oryza sativa L.).联合策略来减轻水稻（Oryza sativa L.）中砷和镉的积累。
Sci Total Environ. 2023 Oct 20;896:165226. doi: 10.1016/j.scitotenv.2023.165226. Epub 2023 Jun 29.
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Rice biofortification: breeding and genomic approaches for genetic enhancement of grain zinc and iron contents.
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水稻生物强化：提高籽粒锌和铁含量的遗传改良育种及基因组方法
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Duplication of a manganese/cadmium transporter gene reduces cadmium accumulation in rice grain.一个锰/镉转运蛋白基因的复制降低了水稻籽粒中的镉积累。
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