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从大肠杆菌染色体中删除末端区域（340千碱基对的DNA）。

Deletion of the terminus region (340 kilobase pairs of DNA) from the chromosome of Escherichia coli.

作者信息

Henson J M, Kuempel P L

出版信息

Proc Natl Acad Sci U S A. 1985 Jun;82(11):3766-70. doi: 10.1073/pnas.82.11.3766.

DOI:10.1073/pnas.82.11.3766
PMID:3889914
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC397868/
Abstract

A strain of Escherichia coli with a 7-minute (340 kilobase pairs of DNA) deletion of the terminus region of the chromosome was isolated. This deletion was probably an IS10-promoted event and its extent was characterized by both genetic and DNA hybridization analyses. The most dramatic property of strains harboring this deletion was the absence of the sites that inhibit clockwise- and counterclockwise-traveling replication forks. These strains also grew slowly, produced many nonviable cells, were filamentous, and appeared to have an induced SOS system.

摘要

分离出了一株大肠杆菌，其染色体末端区域有一段7分钟（340千碱基对DNA）的缺失。这种缺失可能是由IS10引发的事件，其范围通过遗传分析和DNA杂交分析得以确定。携带这种缺失的菌株最显著的特性是缺乏抑制顺时针和逆时针移动复制叉的位点。这些菌株生长也缓慢，产生许多无活力的细胞，呈丝状，并且似乎有一个诱导的SOS系统。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/264e/397868/6013a5e2311e/pnas00351-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/264e/397868/3104d0ed11a4/pnas00351-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/264e/397868/6013a5e2311e/pnas00351-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/264e/397868/3104d0ed11a4/pnas00351-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/264e/397868/6013a5e2311e/pnas00351-0244-a.jpg

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1
Deletion of the terminus region (340 kilobase pairs of DNA) from the chromosome of Escherichia coli.从大肠杆菌染色体中删除末端区域（340千碱基对的DNA）。
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3766-70. doi: 10.1073/pnas.82.11.3766.
2
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A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.大肠杆菌染色体的定义末端区域表现出晚期分离和高 FtsK 活性。
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本文引用的文献

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Improved mapping of the tyrS locus in Escherichia coli.大肠杆菌中tyrS基因座的改进图谱绘制。
Mol Gen Genet. 1980;178(3):647-50. doi: 10.1007/BF00337873.
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Amplification and product identification of the fnr gene of Escherichia coli.大肠杆菌fnr基因的扩增与产物鉴定
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An Escherichia coli mutant defective in the NAD-dependent succinate semialdehyde dehydrogenase.一种在依赖烟酰胺腺嘌呤二核苷酸的琥珀酸半醛脱氢酶方面存在缺陷的大肠杆菌突变体。
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Prophage lambda induces terminal recombination in Escherichia coli by inhibiting chromosome dimer resolution. An orientation-dependent cis-effect lending support to bipolarization of the terminus.λ原噬菌体通过抑制染色体二聚体的解离在大肠杆菌中诱导末端重组。一种依赖方向的顺式效应支持了染色体末端的双极化。
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DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting.通过任意引物PCR（随机扩增多态性DNA）指纹图谱检测大肠杆菌K-12衍生物之间的DNA序列差异。
J Bacteriol. 1994 Mar;176(6):1673-82. doi: 10.1128/jb.176.6.1673-1682.1994.
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The dif resolvase locus of the Escherichia coli chromosome can be replaced by a 33-bp sequence, but function depends on location.大肠杆菌染色体的解离酶基因座可被一个33碱基对的序列取代，但功能取决于其位置。
Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1352-6. doi: 10.1073/pnas.92.5.1352.
7
A cluster of cell division genes maps to the terC region of the chromosome of Escherichia coli K-12.一组细胞分裂基因定位于大肠杆菌K-12染色体的terC区域。
Mol Gen Genet. 1995 Mar 10;246(5):605-9. doi: 10.1007/BF00298967.
8
A new dispensable genetic locus of the terminus region involved in control of cell division in Escherichia coli.大肠杆菌中参与细胞分裂控制的末端区域的一个新的非必需基因座。
Mol Gen Genet. 1985;201(2):146-50. doi: 10.1007/BF00425651.
9
Inhibition of replication forks exiting the terminus region of the Escherichia coli chromosome occurs at two loci separated by 5 min.抑制从大肠杆菌染色体末端区域退出的复制叉发生在两个相隔5分钟的位点。
Proc Natl Acad Sci U S A. 1987 Apr;84(7):1759-63. doi: 10.1073/pnas.84.7.1759.
10
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Proc Natl Acad Sci U S A. 1987 Apr;84(7):1754-8. doi: 10.1073/pnas.84.7.1754.
Arch Microbiol. 1982 Sep;132(3):270-5. doi: 10.1007/BF00407964.
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Cloning and characterization of Escherichia coli K-12 regulator gene tyrR.大肠杆菌K-12调节基因tyrR的克隆与特性分析
J Bacteriol. 1982 Dec;152(3):1276-9. doi: 10.1128/jb.152.3.1276-1279.1982.
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Cloning of trg, a gene for a sensory transducer in Escherichia coli.大肠杆菌中一种感官转导器基因trg的克隆
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Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.大肠杆菌中的诱变作用及对脱氧核糖核酸损伤的诱导反应
Microbiol Rev. 1984 Mar;48(1):60-93. doi: 10.1128/mr.48.1.60-93.1984.
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Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱，第7版。
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Mol Gen Genet. 1984;193(2):263-8. doi: 10.1007/BF00330678.
9
Cloning and physical analysis of the pyrF gene (coding for orotidine-5'-phosphate decarboxylase) from Escherichia coli K-12.来自大肠杆菌K-12的pyrF基因（编码乳清苷-5'-磷酸脱羧酶）的克隆及物理分析。
Gene. 1983 Nov;25(1):39-48. doi: 10.1016/0378-1119(83)90165-8.
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The use of transposon insertion zdc-235::Tn10 (min 32) to clone and delete DNA from the terminus region of Escherichia coli.利用转座子插入zdc - 235::Tn10（最小32）从大肠杆菌末端区域克隆和删除DNA。
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