大肠杆菌染色体的末端区域包含两个独立的位点，它们表现出对复制的极性抑制。
The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication.
作者信息
Hill T M, Henson J M, Kuempel P L
出版信息
Proc Natl Acad Sci U S A. 1987 Apr;84(7):1754-8. doi: 10.1073/pnas.84.7.1754.
Abstract
The terminus region of the chromosome of Escherichia coli contains two separate sites, called T1 and T2, that inhibit replication forks. T1 is located near 28.5 min, which is adjacent to trp, and T2 is located at 34.5-35.7 min on the opposite side of the terminus region, near manA. The sites act in a polar fashion, and replication forks traveling in a clockwise direction with respect to the genetic map are not inhibited as they pass through T1 but are inhibited at T2. Similarly, counterclockwise forks are not inhibited at T2 but are inhibited at T1. Consequently, forks are not inhibited until they have passed through the terminus region and are about to leave it. Studies with deletion strains have located T2 within a 58-kilobase interval, which corresponds to kilobase coordinates 387-445 on the physical map of the terminus region.
摘要
大肠杆菌染色体的末端区域包含两个独立的位点，称为T1和T2，它们会抑制复制叉。T1位于约28.5分钟处，与trp相邻，T2位于末端区域另一侧34.5 - 35.7分钟处，靠近manA。这些位点以极性方式起作用，相对于遗传图谱按顺时针方向移动的复制叉在穿过T1时不会受到抑制，但在T2处会受到抑制。同样，逆时针方向的复制叉在T2处不会受到抑制，但在T1处会受到抑制。因此，复制叉在穿过末端区域并即将离开时才会受到抑制。对缺失菌株的研究已将T2定位在一个58千碱基的区间内，这对应于末端区域物理图谱上的千碱基坐标387 - 445。
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