State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.
Plant Sci. 2024 Sep;346:112159. doi: 10.1016/j.plantsci.2024.112159. Epub 2024 Jun 19.
Wood production is largely determined by the activity of cambial cell proliferation, and the secondary cell wall (SCW) thickening of xylem cells determines the wood property. In this study, we identified an INDETERMINATE DOMAIN (IDD) type C2H2 zinc finger transcription factor PagIDD15A as a regulator of wood formation in Populus alba × Populus glandulosa. Downregulation of PagIDD15A expression by RNA interference (RNAi) inhibited xylem development and xylem cell secondary wall thickening. RNA-seq analysis showed that PagPAL1, PagCCR2 and PagCCoAOMT1 were downregulated in the differentiating xylem of the PagIDD15A-RNAi transgenic plants, showing that PagIDD15A may regulate SCW biosynthesis through inhibiting lignin biosynthesis. The downregulation of PagVND6-B2, PagMYB10 and PagMYC4 and upregulation of PagWRKY12 in the differentiating xylem of RNAi transgenic plants suggest that PagIDD15A may also regulate these transcription factor (TF) genes to affect SCW thickening. RT-qPCR analysis in the phloem-cambium of RNAi transgenic demonstrates that PagIDD15A may regulate the expression of the genes associated with cell proliferation, including, PagSHR (SHORTROOT), PagSCR (SCARECROW), PagCYCD3;1 (CYCLIN D3;1) and PagSMR4 (SIAMESE-RELATED4), to affect the cambial activity. This study provides the knowledge of the IDD-type C2H2 zinc finger protein in regulating wood formation.
木材的产量在很大程度上取决于形成层细胞增殖的活性，而木质部细胞的次生细胞壁（SCW）增厚决定了木材的特性。在这项研究中，我们鉴定出一个不确定域（IDD）类型的 C2H2 锌指转录因子 PagIDD15A，它是胡杨 × 腺柳木质部形成的调节因子。通过 RNA 干扰（RNAi）下调 PagIDD15A 的表达抑制了木质部发育和木质部细胞次生壁的增厚。RNA-seq 分析表明，PagPAL1、PagCCR2 和 PagCCoAOMT1 在 PagIDD15A-RNAi 转基因植物的分化木质部中下调，表明 PagIDD15A 可能通过抑制木质素生物合成来调节 SCW 生物合成。在 RNAi 转基因植物的分化木质部中，PagVND6-B2、PagMYB10 和 PagMYC4 的下调和 PagWRKY12 的上调表明，PagIDD15A 可能还通过调节这些转录因子（TF）基因来影响 SCW 增厚。RNAi 转基因植物韧皮形成层中的 RT-qPCR 分析表明，PagIDD15A 可能通过调节与细胞增殖相关的基因的表达，包括 PagSHR（SHORTROOT）、PagSCR（SCARECROW）、PagCYCD3;1（CYCLIN D3;1）和 PagSMR4（SIAMESE-RELATED4），来影响形成层的活性。本研究为 IDD 型 C2H2 锌指蛋白在调节木材形成中的作用提供了知识。
