Department of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan, Changsha, China.
J Med Entomol. 2024 Sep 10;61(5):1093-1104. doi: 10.1093/jme/tjae071.
Insects and microorganisms, ubiquitous organisms in the natural world, have developed intricate relationships throughout their evolutionary histories. However, most studies have concentrated on specific time points or life stages, but some limited studies have investigated the dynamics of microbial diversity within insects across life stages. Here, 16S rDNA sequencing technology was used to investigate the gut bacterial community across the life stages of Sarcophaga peregrina (Robineau-Desvoidy) (Diptera: Sarcophagidae). The results revealed that the gut bacterial diversity of S. peregrina varied with life stage and showed similarity in the nearby life stages. Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes were the dominant phyla in S. peregrina. Genera such as Providencia, Ignatzschineria, and Myroides are implicated in potentially pivotal roles during the developmental processes of this flesh fly. Furthermore, the effects of amikacin on the growth and development of S. peregrina were not statistically significant. However, we did observe significant changes at the protein level, which suggests a close association between protein-level alterations and growth and development. Additionally, we speculate that S. peregrina regulates its nutritional status during nonfeeding stages to meet the demands of eclosion. This study represents the first comprehensive examination of the intestinal bacterial composition across various life stages of S. peregrina. Our findings deepen our understanding of the gut microbiota in this flesh fly and lay the groundwork for further exploration into the intricate interactions between microorganisms and insects.
昆虫和微生物是自然界中无处不在的生物体，它们在进化历史中形成了复杂的关系。然而，大多数研究都集中在特定的时间点或生命阶段，但有些有限的研究调查了昆虫在整个生命阶段的微生物多样性的动态变化。在这里，16S rDNA 测序技术被用来研究蜉蝣（Sarcophaga peregrina）（双翅目：Sarcophagidae）各生命阶段的肠道细菌群落。结果表明，蜉蝣的肠道细菌多样性随生命阶段而变化，并且在相邻的生命阶段表现出相似性。变形菌门、放线菌门、厚壁菌门和拟杆菌门是蜉蝣的主要门。普罗维登斯菌属、伊格纳齐夏纳菌属和粘球菌属等菌属在这种肉蝇的发育过程中可能具有关键作用。此外，阿米卡星对蜉蝣生长和发育的影响在统计学上并不显著。然而，我们确实观察到了蛋白质水平的显著变化，这表明蛋白质水平的改变与生长和发育密切相关。此外，我们推测蜉蝣在非摄食阶段调节其营养状态以满足出壳的需求。本研究首次全面检查了蜉蝣各生命阶段肠道细菌组成。我们的发现加深了我们对这种肉蝇肠道微生物群的理解，并为进一步探索微生物与昆虫之间的复杂相互作用奠定了基础。
