Taketo A, Taketo Y
Z Naturforsch C Biosci. 1985 Mar-Apr;40(3-4):166-9. doi: 10.1515/znc-1985-3-404.
The amount of streptolysin S produced by resting streptococci was considerably increased after incubation of the washed bacteria with trypsin or pronase. Production of both cell-bound and free forms of the toxin was enhanced by the protease treatment. By addition of trypsin, streptolysin S yield was considerably increased in growing culture as well. Treatment with lysozyme was ineffective, and the toxin production was only slightly promoted by preincubation with hyaluronidase or chymotrypsin. In contrast, pretreatment with chymotrypsin caused increased production of an extracellular nuclease, whereas the yield of this enzyme was reduced after incubation of the cocci with pronase. Evidence was obtained indicating de novo synthesis of the exotoxin in the protease-treated bacteria.
静止的链球菌所产生的链球菌溶血素S的量，在用胰蛋白酶或链霉蛋白酶孵育洗涤过的细菌后显著增加。蛋白酶处理增强了毒素的细胞结合形式和游离形式的产生。通过添加胰蛋白酶，在生长培养物中链球菌溶血素S的产量也显著增加。用溶菌酶处理无效，用透明质酸酶或胰凝乳蛋白酶预孵育仅略微促进毒素产生。相反，用胰凝乳蛋白酶预处理导致细胞外核酸酶产量增加，而球菌与链霉蛋白酶孵育后该酶的产量降低。有证据表明在蛋白酶处理的细菌中外毒素是重新合成的。
