Kuznetsova T A, Kovalevskaia A M, Sanina O P, Besednova N N
Antibiot Med Biotekhnol. 1985 Feb;30(2):109-13.
Study of the effect of lipopolysaccharide (LPS) of Y. pseudotuberculosis on the culture of peritoneal macrophages of guinea pigs has established that in concentrations of 1-10 micrograms/ml LPS stimulated the absorption and digestive activity of the macrophages. In concentrations of 50-100 micrograms/ml it had a cytotoxic effect on the cell elements and inhibited their phagocytic function. The study of the LPS effect on absorption and liberation of 14C-labeled bacteria of Y. pseudotuberculosis showed that the biopolymer increased 2-3 times the uptake of the labeled antigen from the macrophages and lowered its elimination. In the experiments on mice infected with a labeled culture of S. aureus no increase in the uptake under the effect of the pseudotuberculosis LPS was observed, whereas an increase in the rate of the label elimination was stated. The difference was statistically significant. The results of the experiments are indicative of the specific effect of LPS. The study demonstrated a significant role of LPS of Y. pseudotuberculosis in the pathogenesis and protection of the host from this infection.
对假结核耶尔森氏菌脂多糖（LPS）对豚鼠腹腔巨噬细胞培养影响的研究表明，在浓度为1 - 10微克/毫升时，LPS刺激巨噬细胞的吸收和消化活性。在浓度为50 - 100微克/毫升时，它对细胞成分具有细胞毒性作用并抑制其吞噬功能。对LPS对假结核耶尔森氏菌14C标记细菌的吸收和释放影响的研究表明，该生物聚合物使巨噬细胞对标记抗原的摄取增加2 - 3倍，并降低其清除率。在用金黄色葡萄球菌标记培养物感染小鼠的实验中，未观察到假结核LPS作用下摄取增加，而标记物清除率增加。差异具有统计学意义。实验结果表明LPS具有特异性作用。该研究证明假结核耶尔森氏菌LPS在宿主针对这种感染的发病机制和保护中起重要作用。
