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[用半胱氨酸抗坏血酸作为光镜和电镜组织学材料固定剂的应用]

[Use of galascorbin as a fixative of histological material for light and electron microscopy].

作者信息

Shavrin V A, Polkovnikov Iu F

出版信息

Arkh Patol. 1985;47(3):73-6.

PMID:3890809
Abstract

A complex of sodium salts of gallic and ascorbic acids (galascorbin) is recommended as an additive to the fixative for improving the quality of histological material fixation. Its main advantage as compared to natural tannids is an easiness of penetration into the depth of tissues and, in comparison to other fixatives (aldehydes, alcohol), a more efficient fixation and retention of intra- and extracellular substances in tissues. Prescriptions of the galascorbic fixatives for light and electron microscopy are given. A method of staining of preparations specifically adapted for galascorbic fixation is suggested for light microscopy. Methods of both fixation and staining are intended for scientific purposes and for use by prosectors.

摘要

没食子酸和抗坏血酸钠盐复合物(没食子抗坏血酸)被推荐作为固定剂的添加剂,以提高组织学材料固定的质量。与天然单宁相比,其主要优点是易于渗透到组织深处,与其他固定剂(醛类、酒精)相比,能更有效地固定和保留组织中的细胞内和细胞外物质。给出了用于光学显微镜和电子显微镜的没食子抗坏血酸固定剂的配方。针对光学显微镜,提出了一种专门适用于没食子抗坏血酸固定的制片染色方法。固定和染色方法均用于科学目的,供解剖人员使用。

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