Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Ultimo, NSW 2007, Australia; Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, NSW 2007, Australia; Pharmako Biotechnologies, Frenchs Forest, NSW 2086, Australia.
Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Ultimo, NSW 2007, Australia; Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, NSW 2007, Australia.
Pathol Res Pract. 2024 Aug;260:155423. doi: 10.1016/j.prp.2024.155423. Epub 2024 Jun 20.
Curcumin, the principal curcuminoid of turmeric (Curcuma longa extract), is very well known for its multiple biological therapeutic activities, particularly its anti-inflammatory and antioxidant potential. However, due to its low water solubility, it exhibits poor bioavailability. In order to overcome this problem, in the current study, we have employed liposomal technology to encapsulate curcumin with the aim of enhancing its therapeutic efficacy. The curcumin-loaded liposomes (PlexoZome®) were tested on a cigarette smoke extract-induced Chronic Obstructive Pulmonary Disease (COPD) in vitro model using minimally immortalized human bronchial epithelial cells (BCiNS1.1). The anti-senescence and anti-inflammatory properties of PlexoZome® were explored. 5 µM PlexoZome® curcumin demonstrated anti-senescent activity by decrease in X-gal positive cells, and reduction in the expression of p16 and p21 in immunofluorescence staining. Moreover, PlexoZome® curcumin also demonstrated a reduction in proteins related to senescence (osteopontin, FGF basic and uPAR) and inflammation (GM-CSF, EGF and ST2). Overall, the results clearly demonstrate the therapeutic potential of curcumin encapsulated liposomes in managing CSE induced COPD, providing a new direction to respiratory clinics.
姜黄素是姜黄（姜黄提取物）中的主要姜黄素类化合物，因其多种生物治疗活性而广为人知，特别是其抗炎和抗氧化潜力。然而，由于其低水溶性，其生物利用度较差。为了克服这个问题，在目前的研究中，我们采用脂质体技术将姜黄素包封，旨在提高其治疗效果。用载有姜黄素的脂质体（PlexoZome®）对体外香烟烟雾提取物诱导的慢性阻塞性肺疾病（COPD）模型进行了测试，该模型使用最小永生化的人支气管上皮细胞（BCiNS1.1）。研究了 PlexoZome®的抗衰老和抗炎特性。5µMPlexoZome®姜黄素通过减少 X-gal 阳性细胞，以及通过免疫荧光染色减少 p16 和 p21 的表达，表现出抗衰老活性。此外，PlexoZome®姜黄素还减少了与衰老（骨桥蛋白、碱性成纤维细胞生长因子和 uPAR）和炎症（GM-CSF、EGF 和 ST2）相关的蛋白质。总的来说，这些结果清楚地表明了包封在脂质体中的姜黄素在管理香烟烟雾提取物诱导的 COPD 中的治疗潜力，为呼吸科诊所提供了一个新的方向。
