Department of Otorhinolaryngology, Bezmialem Vakif University, Faculty of Medicine, Istanbul, Turkey.
Department of Otorhinolaryngology, Bezmialem Vakif University, Faculty of Medicine, Istanbul, Turkey.
J Oral Maxillofac Surg. 2024 Sep;82(9):1067-1075. doi: 10.1016/j.joms.2024.05.041. Epub 2024 Jun 10.
Although rhinoplastic surgery has progressed considerably in recent years, nasal dorsal irregularities still cause postoperative distress for both surgeons and patients.
The aim of this study was to measure the association between two biologic graft adjuncts, platelet-rich plasma (PRP) and concentrated growth factor (CGF), and ultra-diced cartilage viability in an animal model.
STUDY DESIGN, SETTING, AND SAMPLE: This study was designed as a randomized in-vivo study using a rabbit model. Fourteen rabbits were utilized in this investigation. The ultra-diced cartilage was obtained from auricular cartilage.
The graft biologic adjunct is the predictor variable. There were three treatment groups: graft mixed with PRP or CGF or untreated (control). The grafts were placed in three separate pockets opened on the same rabbit. Grafts were harvested 3 months after insertion for analysis.
MAIN OUTCOME VARIABLE(S): The primary outcome variable was histopathological and regenerative scores obtained from multiple histopathological parameters indicating the viability of the cartilage. Histopathological score parameters were chondrocyte loss, inflammation, fibrosis, cartilage fragmentation, and calcified area formations in the lacunae. Regenerative score parameters were peripheral cell proliferation in the cartilage tissue, vascularization in the connective tissue, proteoglycan increase in the matrix, and the amount of connective tissue.
The variables were age, sex, and weight.
Statistical analysis employed the analysis of variance test, with a significance level of P < .05.
The sample was composed of 14 rabbits and 42 samples. The histopathologic scores were 11.93 (±2.49), 8.78 (±2.19), and 6.85 (±1.46) for the control, PRP, and CGF groups, respectively. A statistically significant difference was found in the PRP (P < .0275) and CGF (P < .0001) groups compared to the control group. The regenerative scores were 6.21 (±0.97), 8.85 (±1.70), and 12.07 (±1.26) for the control, PRP and CGF groups, respectively. A statistically significant difference was found in the PRP (P < .0159) and CGF (P < .0001) groups compared to the control group.
This is the first study investigating the ultra-diced cartilage graft in an experimental animal model. Histopathological examination has shown that mixing ultra-diced cartilage with CGF or PRP increases viability by reducing the histopathological score and increasing the regenerative score.
尽管近年来鼻整形术取得了长足的进步，但鼻背畸形仍然给外科医生和患者带来术后困扰。
本研究旨在测量两种生物移植物辅助物——富血小板血浆（PRP）和浓缩生长因子（CGF）与超微软骨活力之间的关联。
研究设计、地点和样本：本研究设计为使用兔模型的随机体内研究。本研究共使用了 14 只兔子。超微软骨取自耳廓软骨。
移植物生物辅料是预测变量。有三个治疗组：与 PRP 或 CGF 混合的移植物或未处理的（对照）移植物。将移植物放置在同一只兔子上打开的三个单独口袋中。植入 3 个月后取出移植物进行分析。
主要结局变量是从多个组织病理学参数获得的组织病理学和再生评分，这些参数表明软骨的活力。组织病理学评分参数包括软骨细胞丢失、炎症、纤维化、软骨碎裂和陷窝内钙化区形成。再生评分参数包括软骨组织中的周围细胞增殖、结缔组织中的血管生成、基质中糖胺聚糖的增加以及结缔组织的量。
变量为年龄、性别和体重。
统计分析采用方差分析检验，显著性水平 P < 0.05。
样本由 14 只兔子和 42 个样本组成。对照组、PRP 组和 CGF 组的组织病理学评分分别为 11.93（±2.49）、8.78（±2.19）和 6.85（±1.46）。与对照组相比，PRP 组（P < 0.0275）和 CGF 组（P < 0.0001）的组织病理学评分有统计学意义。对照组、PRP 组和 CGF 组的再生评分分别为 6.21（±0.97）、8.85（±1.70）和 12.07（±1.26）。与对照组相比，PRP 组（P < 0.0159）和 CGF 组（P < 0.0001）的再生评分有统计学意义。
这是第一项在实验动物模型中研究超微软骨移植物的研究。组织病理学检查表明，通过降低组织病理学评分和增加再生评分，将超微软骨与 CGF 或 PRP 混合可提高软骨活力。
