College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China.
Anticancer Agents Med Chem. 2024;24(15):1151-1158. doi: 10.2174/0118715206299886240620070011.
Chronic myelogenous leukemia (CML) is an uncommon type of cancer of the bone marrow associated with high mortality. Although several effective therapies have been developed to reduce symptoms in patients with CML, many of these methods are associated with side effects. Nutt. () is a natural medicinal material that possesses antioxidant and anticancer activities. Yet, its effect in treating leukemia has still not been fully explored.
To optimize the flower extraction process and investigate whether these extracts can impair CML cell survival.
The extraction process of was optimized by the Box-Behnken design response surface method. K562 cells were treated with different volumes (0, 10, 25, 50, and 100 μL) of flower extracts. The effect of extract on cell morphology and cell apoptosis was assessed by light microscopy, laser confocal microscopy, and flow cytometry.
We established the following optimized flower extraction conditions: temperature of 84.4°C, extraction period of 10 mins, solid-liquid ratio of 1:65, and times 4. These conditions were applied for flower extraction. Pre-incubation of extracts prepared under the aforementioned optimal conditions with K562 cells induced cell cytotoxicity and cell apoptosis.
flower extracts exert obvious anti-leukemia effects in vitro and may be a potential drug candidate for leukemia treatment.
慢性髓性白血病（CML）是一种罕见的骨髓癌，死亡率较高。尽管已经开发出几种有效的疗法来减轻 CML 患者的症状，但这些方法中的许多都与副作用相关。五味子是一种具有抗氧化和抗癌活性的天然药材。然而，其在治疗白血病方面的作用尚未得到充分探索。
优化五味子花的提取工艺，并研究其提取物是否能损害 CML 细胞的存活。
采用 Box-Behnken 设计响应面法优化五味子花的提取工艺。用不同体积（0、10、25、50 和 100μL）的五味子花提取物处理 K562 细胞。通过光镜、激光共聚焦显微镜和流式细胞术评估五味子提取物对细胞形态和细胞凋亡的影响。
建立了以下优化的五味子花提取条件：温度 84.4°C，提取时间 10 分钟，固液比 1:65，提取次数 4。在上述最佳条件下提取的提取物预孵育 K562 细胞可诱导细胞毒性和细胞凋亡。
五味子花提取物在体外具有明显的抗白血病作用，可能是治疗白血病的潜在药物候选物。
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