Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Xueyuan Street, Xiasha Higher Education Zone, Hangzhou 310018, China.
Qianjiang Customs of the People's Republic of China, Hangzhou 310012, China.
Biosensors (Basel). 2024 Jun 4;14(6):292. doi: 10.3390/bios14060292.
Olaquindox (OLA) and quinocetone (QCT) have been prohibited in aquatic products due to their significant toxicity and side effects. In this study, rapid and visual europium nanoparticle (EuNP)-based lateral flow strip biosensors (LFSBs) were developed for the simultaneous quantitative detection of OLA, QCT, and 3-methyl-quinoxaline-2-carboxylic acid (MQCA) in fish feed and tissue. The EuNP-LFSBs enabled sensitive detection for OLA, QCT, and MQCA with a limit of detection of 0.067, 0.017, and 0.099 ng/mL (R ≥ 0.9776) within 10 min. The average recovery of the EuNP-LFSBs was 95.13%, and relative standard deviations were below 9.38%. The method was verified by high-performance liquid chromatography (HPLC), and the test results were consistent. Therefore, the proposed LFSBs serve as a powerful tool to monitor quinoxalines in fish feeds and their residues in fish tissues.
奥拉喹酮（OLA）和喹乙醇（QCT）由于其毒性和副作用大，已被禁止在水产养殖中使用。在这项研究中，开发了基于铕纳米粒子（EuNP）的快速可视化侧向流动条生物传感器（LFSB），用于同时定量检测鱼饲料和组织中的 OLA、QCT 和 3-甲基喹喔啉-2-羧酸（MQCA）。EuNP-LFSB 对 OLA、QCT 和 MQCA 的检测具有较高的灵敏度，检出限分别为 0.067、0.017 和 0.099ng/mL（R≥0.9776），在 10 分钟内完成检测。EuNP-LFSB 的平均回收率为 95.13%，相对标准偏差低于 9.38%。该方法通过高效液相色谱（HPLC）进行了验证，测试结果一致。因此，该 LFSB 可作为监测鱼类饲料中喹噁啉及其在鱼类组织中残留的有效工具。
