基于铕纳米粒子的侧向流条生物传感器用于检测鱼饲料和组织中的喹喔啉类抗生素及其主要代谢物。
Europium Nanoparticle-Based Lateral Flow Strip Biosensors for the Detection of Quinoxaline Antibiotics and Their Main Metabolites in Fish Feeds and Tissues.
机构信息
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Xueyuan Street, Xiasha Higher Education Zone, Hangzhou 310018, China.
Qianjiang Customs of the People's Republic of China, Hangzhou 310012, China.
出版信息
Biosensors (Basel). 2024 Jun 4;14(6):292. doi: 10.3390/bios14060292.
Olaquindox (OLA) and quinocetone (QCT) have been prohibited in aquatic products due to their significant toxicity and side effects. In this study, rapid and visual europium nanoparticle (EuNP)-based lateral flow strip biosensors (LFSBs) were developed for the simultaneous quantitative detection of OLA, QCT, and 3-methyl-quinoxaline-2-carboxylic acid (MQCA) in fish feed and tissue. The EuNP-LFSBs enabled sensitive detection for OLA, QCT, and MQCA with a limit of detection of 0.067, 0.017, and 0.099 ng/mL (R ≥ 0.9776) within 10 min. The average recovery of the EuNP-LFSBs was 95.13%, and relative standard deviations were below 9.38%. The method was verified by high-performance liquid chromatography (HPLC), and the test results were consistent. Therefore, the proposed LFSBs serve as a powerful tool to monitor quinoxalines in fish feeds and their residues in fish tissues.
奥拉喹酮（OLA）和喹乙醇（QCT）由于其毒性和副作用大，已被禁止在水产养殖中使用。在这项研究中，开发了基于铕纳米粒子（EuNP）的快速可视化侧向流动条生物传感器（LFSB），用于同时定量检测鱼饲料和组织中的 OLA、QCT 和 3-甲基喹喔啉-2-羧酸（MQCA）。EuNP-LFSB 对 OLA、QCT 和 MQCA 的检测具有较高的灵敏度，检出限分别为 0.067、0.017 和 0.099ng/mL（R≥0.9776），在 10 分钟内完成检测。EuNP-LFSB 的平均回收率为 95.13%，相对标准偏差低于 9.38%。该方法通过高效液相色谱（HPLC）进行了验证，测试结果一致。因此，该 LFSB 可作为监测鱼类饲料中喹噁啉及其在鱼类组织中残留的有效工具。
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