Xie Meng, Yao Yuhao, Feng Yuling, Xie Lei, Mao Chuyang, He Jinwu, Li Xueyan, Ni Qingyong
College of Life Science, Sichuan Agricultural University, Ya'an 625014, China.
Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Insects. 2024 Jun 6;15(6):431. doi: 10.3390/insects15060431.
Attelabidae insects have attracted much attention due to their unique leaf rolling behavior before oviposition. However, the lack of genomic data makes it difficult to understand the molecular mechanism behind their behavior and their evolutionary relationship with other species. To address this gap, we utilized Illumina and Nanopore sequencing platforms along with Hi-C technology to establish a highly accurate whole genome of at the chromosome level. The resulting genome size was determined to be 619.26 Mb, with a contig N50 of 50.89 Mb and GC content of 33.89%. Moreover, a total of 12,572 genes were identified, with 82.59% being functionally annotated, and 64.78% designated as repeat sequences. Our subsequent phylogenetic tree analysis revealed that Attelabidae's divergence from Curculionidae occurred approximately 161.52 million years ago. Furthermore, the genome of contained 334 expanded gene families and 1718 contracted gene families. In addition, using Phylogenetic Analysis by Maximum Likelihood (PAML), we identified 106 rapidly evolved genes exhibiting significant signals and 540 positively selected genes. Our research endeavors to serve as an invaluable genomic data resource for the study of Attelabidae, offering fresh perspectives for the exploration of its leaf rolling behavior.
卷叶象甲科昆虫因其独特的产卵前卷叶行为而备受关注。然而，由于缺乏基因组数据，难以了解其行为背后的分子机制以及它们与其他物种的进化关系。为填补这一空白，我们利用Illumina和Nanopore测序平台以及Hi-C技术，在染色体水平上建立了一个高度准确的全基因组。测定所得基因组大小为619.26 Mb，重叠群N50为50.89 Mb，GC含量为33.89%。此外，共鉴定出12572个基因，其中82.59%具有功能注释，64.78%被指定为重复序列。我们随后的系统发育树分析表明，卷叶象甲科与象甲科的分化大约发生在1.6152亿年前。此外，该基因组包含334个扩张基因家族和1718个收缩基因家族。此外，使用最大似然法系统发育分析（PAML），我们鉴定出106个显示出显著信号的快速进化基因和540个正选择基因。我们的研究致力于为卷叶象甲科的研究提供宝贵的基因组数据资源，为探索其卷叶行为提供新的视角。
