Hazzouri Khaled Michel, Sudalaimuthuasari Naganeeswaran, Kundu Biduth, Nelson David, Al-Deeb Mohammad Ali, Le Mansour Alain, Spencer Johnston J, Desplan Claude, Amiri Khaled M A
Khalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, PO Box 15551, Al Ain, UAE.
Department of Biology, United Arab Emirates University, PO Box 15551, Al Ain, UAE.
Commun Biol. 2020 Jun 24;3(1):323. doi: 10.1038/s42003-020-1060-8.
The red palm weevil, Rhynchophorus ferrugineus, infests palm plantations, leading to large financial losses and soil erosion. Pest-host interactions are poorly understood in R. ferrugineus, but the analysis of genetic diversity and pest origins will help advance efforts to eradicate this pest. We sequenced the genome of R. ferrugineus using a combination of paired-end Illumina sequencing (150 bp), Oxford Nanopore long reads, 10X Genomics and synteny analysis to produce an assembly with a scaffold N50 of ~60 Mb. Structural variations showed duplication of detoxifying and insecticide resistance genes (e.g., glutathione S-transferase, P450, Rdl). Furthermore, the evolution of gene families identified those under positive selection including one glycosyl hydrolase (GH16) gene family, which appears to result from horizontal gene transfer. This genome will be a valuable resource to understand insect evolution and behavior and to allow the genetic modification of key genes that will help control this pest.
红棕象甲（Rhynchophorus ferrugineus）会侵扰棕榈种植园，导致巨大的经济损失和土壤侵蚀。目前人们对红棕象甲与宿主之间的相互作用了解甚少，但对其遗传多样性和害虫起源的分析将有助于推动根除这种害虫的工作。我们结合使用双末端Illumina测序（150 bp）、牛津纳米孔长读长测序、10X基因组学和共线性分析对红棕象甲的基因组进行了测序，以产生一个支架N50约为60 Mb的组装体。结构变异显示出解毒和抗杀虫剂基因（如谷胱甘肽S-转移酶、P450、Rdl）的重复。此外，基因家族的进化鉴定出了处于正选择下的基因家族，包括一个糖基水解酶（GH16）基因家族，这似乎是水平基因转移的结果。该基因组将成为理解昆虫进化和行为以及对有助于控制这种害虫的关键基因进行遗传修饰的宝贵资源。
Zotero 插件