外周血中甲基化与肺癌的关联：一项病例对照研究。

Association between the methylations of in peripheral blood and lung cancer: a case-control study.

机构信息

Research and Development Department, TANTICA Biotechnology (Shanghai) Co., Ltd, Shanghai, China.

Department of Clinical Laboratory, Jiangsu Province Hospital of Chinese Medicine, Nanjing, China.

出版信息

Biomarkers. 2024 Sep;29(6):343-351. doi: 10.1080/1354750X.2024.2373714. Epub 2024 Jul 23.

DOI:10.1080/1354750X.2024.2373714

PMID:38923933

Abstract

BACKGROUND

is hypermethylated in multiple cancers. also functions as a regulator of tumors. However, there are only very few reports on the association of methylation of and with lung cancer (LC) in peripheral blood.

METHODS

426 LC patients and 428 age- and sex-matched healthy controls were recruited. DNA methylation in blood was semi-quantitively assessed by mass spectrometry. For the association analysis, binary logistic regression analysis adjusted covariant was applied, and ORs were presented as per +10% methylation.

RESULTS

Hypermethylation of CpG_1, CpG_5 and CpG_8 in was significantly associated with LC (ORs = 1.45, 1.35 and 1.35, respectively, adjusted < 0.05), and even stage I LC. The association between the three CpG sites and LC was enhanced by increased age (> 55 years, ORs ranged from 1.43 to 1.75, adjusted < 0.05), male gender (ORs ranged from 1.47 to 1.59, adjusted < 0.05) and tumor stage (stage II&III&IV, ORs ranged from 1.86 to 3.03, adjusted < 0.05).

CONCLUSIONS

This study suggests a significant association between blood-based hypermethylation and LC, especially in elder people, in males and in LC patients with advanced stage.

摘要

背景

在多种癌症中呈超甲基化状态。同时也作为肿瘤的调节剂。然而，关于血液中甲基化与肺癌（LC）的关联，仅有极少数报道。

方法

招募了 426 名 LC 患者和 428 名年龄和性别匹配的健康对照者。通过质谱法对半定量评估血液中的 DNA 甲基化。对于关联分析，应用了调整协变量的二元逻辑回归分析，并以每 +10%甲基化的 OR 呈现。

结果

CpG_1、CpG_5 和 CpG_8 中的超甲基化与 LC（ORs=1.45、1.35 和 1.35，分别为调整后 <0.05）显著相关，甚至在 I 期 LC 中也是如此。这三个 CpG 位点与 LC 之间的关联随着年龄的增加（>55 岁，OR 范围从 1.43 到 1.75，调整后 <0.05）、男性性别（OR 范围从 1.47 到 1.59，调整后 <0.05）和肿瘤分期（II&III&IV 期，OR 范围从 1.86 到 3.03，调整后 <0.05）而增强。