Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin, ul. Skromna 8, Lublin, 20-704, Poland.
Department of Bioanalytics, Medical University of Lublin, ul. Jaczewskiego 8b, Lublin, 20-090, Poland.
Mol Nutr Food Res. 2024 Jul;68(13):e2300426. doi: 10.1002/mnfr.202300426. Epub 2024 Jun 24.
Bilberry, bananas, and apples are used for smoothie production because the health-promoting activities and to prevent human diseases including neurodegenerative disorders. The smoothie is prepared to promote a promising practice for increasing the intake of fruit in the diet.
The smoothie is packed into dark glass jars, pasteurized, and stored for up to 4 months at 4 or 22 °C. Then, it is analyzed for the polyphenols profile using liquid chromatography-high resolution mass spectometry (LC-HRMS) Polyphenols content and the antiinflammatory, anticholinesterase, and antioxidant activities, and the impact on catalase activity are controlled using biochemical analyses. A significant decrease in the flavanol content (p < 0.05) is investigated, while there are lower decreases or no changes in the other polyphenols content in the smoothies stored at 4 °C. The changes in the anticholinesterase and antioxidant activities of the smoothie are correlated with the total polyphenols, anthocyanins, flavonols, and tannins content.
The proposed preservation of the smoothie and its storage at refrigeration temperature is adequate to maintain the smoothie's nutritional and functional effect for a 4-month shelf life. Even significant changes in the content of individual subgroups of polyphenols are not drastically reflected in the decrease of the smoothie biological activities.
越橘、香蕉和苹果用于制作冰沙，因为它们具有促进健康的活动，可预防包括神经退行性疾病在内的人类疾病。这种冰沙的制备旨在促进增加饮食中水果摄入量的有前景的做法。
冰沙装在深色玻璃罐中，巴氏杀菌，并在 4 或 22°C 下储存长达 4 个月。然后，使用液相色谱-高分辨率质谱法 (LC-HRMS) 分析其多酚谱，使用生化分析控制多酚含量以及抗炎、抗胆碱酯酶和抗氧化活性，以及对过氧化氢酶活性的影响。研究发现， flavanol 含量显著下降（p < 0.05），而在 4°C 下储存的冰沙中其他多酚含量下降较小或没有变化。冰沙的抗胆碱酯酶和抗氧化活性的变化与总多酚、花青素、类黄酮和单宁含量相关。
建议对冰沙进行保存，并在冷藏温度下储存，以在 4 个月的保质期内保持冰沙的营养和功能效果。即使多酚类物质的个别亚组含量发生显著变化，也不会明显反映在冰沙生物活性的降低上。
