Teikyo University Institute of Medical Mycology (TIMM), Tokyo, Japan.
Veterinary Hospital Aichi P.F.A.M.A.A., Aichi, Japan.
J Vet Med Sci. 2024 Aug 2;86(8):847-849. doi: 10.1292/jvms.24-0032. Epub 2024 Jul 4.
Phytochemicals isolated from essential oils are effective alternatives for inhibiting microbial pathogens. Bovine protothecal mastitis is the cause of a reduction in milk production and the secretion of thin, watery milk with white flakes. In the present study, we performed in vitro susceptibility testing of the phytochemicals carvacrol, citral, and thymol in Prototheca strains isolated from cases of protothecosis in small animals and cow feces. The susceptibility of the algae to carvacrol, citral, and thymol was assessed using the modified CLSI M27-A3 broth microdilution method. The ranges of the minimum inhibitory concentrations (MIC%) of the phytochemicals in all isolates were 0.03% to 0.125% for carvacrol, 0.03% to 0.25% for citral, and 0.06% to 0.25% for thymol. Based on these results, carvacrol, citral, and thymol appear effective against Prototheca species at the tested concentrations, and may thus be useful for environmental disinfection in barns.
从精油中分离出的植物化学物质是抑制微生物病原体的有效替代品。牛原浆性乳腺炎是导致产奶量减少和分泌稀薄、水样、带有白色片状物的乳汁的原因。在本研究中，我们对从小动物和牛粪便中原位分离的原浆菌属菌株进行了植物化学物质香芹酚、柠檬醛和百里香酚的体外药敏试验。使用改良的 CLSI M27-A3 肉汤微量稀释法评估藻类对香芹酚、柠檬醛和百里香酚的敏感性。所有分离株对植物化学物质香芹酚、柠檬醛和百里香酚的最小抑菌浓度（MIC%）范围分别为 0.03%至 0.125%、0.03%至 0.25%和 0.06%至 0.25%。基于这些结果，香芹酚、柠檬醛和百里香酚在测试浓度下对原浆菌属似乎有效，因此可能有助于牛舍的环境消毒。
