Wright N G, Mohammed N A, Eckersall P D, Nash A S
Res Vet Sci. 1985 May;38(3):322-8.
Eight 16-week-old dogs were used to induce immune complex glomerulonephritis by daily intravenous injections of 120 mg highly cationized bovine serum albumin (pI9.5). Of four control dogs, two received unmodified native anionic bovine serum albumin (pI 4.5) while the other two received only phosphate buffered saline. The renal glomeruli were examined by light, electron (transmission and scanning) and immunofluorescence microscopy at intervals from five to 11 weeks after the start of the injections. Animals receiving cationic antigen all developed generalised diffuse granular deposits of IgG and C3 along the capillary walls; these were detected as early as five weeks and continued until the termination of the experiment at 11 weeks. Ultrastructural studies revealed many electron dense deposits along the subepithelial regions of the glomerular basement membrane. The experimental disease resembled in many respects naturally occurring membranous nephropathy, the most common form of immune complex glomerulonephritis in dogs.
选用8只16周龄的犬，通过每日静脉注射120毫克高度阳离子化牛血清白蛋白（pI9.5）诱导免疫复合物性肾小球肾炎。4只对照犬中，2只接受未修饰的天然阴离子牛血清白蛋白（pI 4.5），另外2只只接受磷酸盐缓冲盐水。在注射开始后的5至11周内，定期通过光学显微镜、电子显微镜（透射和扫描）以及免疫荧光显微镜检查肾小球。接受阳离子抗原的动物均在毛细血管壁上出现IgG和C3的全身性弥漫性颗粒状沉积物；最早在5周时即可检测到，并且一直持续到实验在11周结束。超微结构研究显示，在肾小球基底膜的上皮下区域有许多电子致密沉积物。该实验性疾病在许多方面类似于自然发生的膜性肾病，这是犬免疫复合物性肾小球肾炎最常见的形式。
