Wu Chang-Cheng, Ding De-Sing, Lo Yi-Hao, Pan Chieh-Yu, Wen Zhi-Hong
Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan.
Department of Obstetrics and Gynecology, Zuoying Armed Forces General Hospital, Kaohsiung 81342, Taiwan.
Biology (Basel). 2024 May 27;13(6):384. doi: 10.3390/biology13060384.
is a seaweed rich in polysaccharides often used in food, feed, fertilizers, and antibacterial drugs. This study is the first to evaluate the effect of feeding zebrafish with extract on preventing and treating infections. This study evaluated the growth, survival, and disease resistance effects of extract on zebrafish. The fish were divided into four groups: three groups treated with 1%, 5%, or 10% extract and one untreated group (c, control). Subsequently, we analyzed how the extract affected the immune function of zebrafish infected with . Based on the lethal concentration (LC50) calculated in the first stage, 1% was used as the effective therapeutic concentration. The results showed that the growth rate of the 1% feed group was the best, and no significant difference in survival rates between the four groups was observed. Feeding with 1% extract downregulated the expression of key inflammatory genes like tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), and IL-10, effectively preventing and treating infections in zebrafish. This study is a preliminary evaluation of the therapeutic efficacy of extracts against .
是一种富含多糖的海藻，常用于食品、饲料、肥料和抗菌药物中。本研究首次评估了用提取物喂养斑马鱼对预防和治疗感染的效果。本研究评估了提取物对斑马鱼的生长、存活和抗病能力的影响。将鱼分为四组：三组分别用1%、5%或10%的提取物处理，一组未处理（c，对照组）。随后，我们分析了提取物如何影响感染的斑马鱼的免疫功能。根据第一阶段计算的致死浓度（LC50），将1%用作有效治疗浓度。结果表明，1%饲料组的生长速率最佳，四组之间的存活率未观察到显著差异。用1%的提取物喂养可下调关键炎症基因如肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和IL-10的表达，有效预防和治疗斑马鱼的感染。本研究是对提取物抗治疗效果的初步评估。
