Wattanathorn Jintanaporn, Thukham-Mee Wipawee
Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Research Institute for High Human Performance and Health Promotion, Khon Kaen University, Khon Kaen 40002, Thailand.
Antioxidants (Basel). 2024 May 24;13(6):637. doi: 10.3390/antiox13060637.
To increase the value of the by-products of the canned tuna industry, the memory enhancement effect and the possible mechanisms of omega-3-rich tuna oil in bilateral ovariectomized (OVX) rats were assessed. Female rats were orally given tuna oil at doses of 140, 200, and 250 mg/kg of body weight (BW) for 28 days before OVX and for 21 days continually after OVX. Memory performance was assessed every week, whereas the parameters regarding mechanisms of action were assessed at the end of the study. All doses of tuna oil enhanced memory, docosahexaenoic acid (DHA) levels, and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities but decreased cortisol, acetylcholinesterase (AChE), malondialdehyde (MDA), and inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Medium and high doses of tuna oil suppressed monoamine oxidase (MAO) but increased eNOS activity. A high dose of tuna oil suppressed gamma-aminotransferase (GABA-T) but increased glutamic acid decarboxylase (GAD) and sirtuin-1. A medium dose of tuna oil decreased homocysteine (Hcys) and C-reactive protein. No change in telomere or estradiol was observed in this study. Our results suggest the memory-enhancing effect of tuna oil in an OVX rat model of menopause. The main mechanisms may involve a reduction in oxidative stress, inflammation, and neurotransmitter regulation.
为提高罐装金枪鱼产业副产品的价值，评估了富含omega-3的金枪鱼油对双侧卵巢切除（OVX）大鼠的记忆增强作用及其可能机制。雌性大鼠在卵巢切除前28天和卵巢切除后连续21天，分别按140、200和250mg/kg体重的剂量口服金枪鱼油。每周评估记忆表现，而在研究结束时评估有关作用机制的参数。所有剂量的金枪鱼油均能增强记忆、提高二十二碳六烯酸（DHA）水平、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性，但降低皮质醇、乙酰胆碱酯酶（AChE）、丙二醛（MDA）以及肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）等炎性细胞因子。中、高剂量的金枪鱼油抑制单胺氧化酶（MAO）但增加内皮型一氧化氮合酶（eNOS）活性。高剂量的金枪鱼油抑制γ-氨基转移酶（GABA-T）但增加谷氨酸脱羧酶（GAD）和沉默信息调节因子1。中剂量的金枪鱼油降低同型半胱氨酸（Hcys）和C反应蛋白。本研究未观察到端粒或雌二醇的变化。我们的结果表明金枪鱼油对绝经后OVX大鼠模型具有记忆增强作用。主要机制可能包括减轻氧化应激、炎症和调节神经递质。
