Perry Matthew J, Curic Mara, Scott Abigail L, Ritmejerytė Edita, Rahayu Dyah U C, Keller Paul A, Oelgemöller Michael, Yeshi Karma, Wangchuk Phurpa
College of Public Health, Medical and Veterinary Sciences, James Cook University, Cairns, QLD 4878, Australia.
Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD 4878, Australia.
Life (Basel). 2024 May 30;14(6):710. doi: 10.3390/life14060710.
Recent studies have shown that seagrasses could possess potential applications in the treatment of inflammatory disorders. Five seagrass species (, , , , and ) from the Great Barrier Reef (QLD, Australia) were thus collected, and their preliminary antioxidant and anti-inflammatory activities were evaluated. From the acetone extracts of five seagrass species subjected to 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging antioxidant assay, the extract of had the highest activity (half minimal concentration of inhibition (IC) = 138 µg/mL), with the aerial parts (IC = 119 µg/mL) possessing significantly higher antioxidant activity than the roots (IC ≥ 500 µg/mL). A human peripheral blood mononuclear cells (PBMCs) assay with bacterial lipopolysaccharide (LPS) activation and LEGENDplex cytokine analysis showed that the aerial extract of significantly reduced the levels of inflammatory cytokines tumour necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 by 29%, 74%, and 90%, respectively, relative to the LPS treatment group. The aerial extract was thus fractionated with methanol (MeOH) and hexane fraction, and purification of the MeOH fraction by HPLC led to the isolation of 4-hydroxybenzoic acid (), luteolin (), and apigenin () as its major constituents. These compounds have been previously shown to reduce levels of TNF-α, IL-1β, and IL-6 and represent some of the major bioactive components of aerial parts. This investigation represents the first study of the antioxidant and anti-inflammatory properties of and the first isolation of small molecules from this species. These results highlight the potential for using seagrasses in treating inflammation and the need for further investigation.
最近的研究表明，海草在炎症性疾病的治疗中可能具有潜在应用价值。因此，采集了来自澳大利亚昆士兰州大堡礁的5种海草（、、、和），并对其初步的抗氧化和抗炎活性进行了评估。在对5种海草的丙酮提取物进行1,1-二苯基-2-苦基肼（DPPH）自由基清除抗氧化测定中，的提取物活性最高（半数抑制浓度（IC）=138微克/毫升），其地上部分（IC=119微克/毫升）的抗氧化活性显著高于根部（IC≥500微克/毫升）。一项使用细菌脂多糖（LPS）激活的人外周血单核细胞（PBMCs）测定和LEGENDplex细胞因子分析表明，相对于LPS处理组，的地上提取物分别使炎症细胞因子肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-1β和IL-6的水平显著降低了29%、74%和90%。因此，将地上提取物用甲醇（MeOH）和己烷进行分级分离，通过高效液相色谱法（HPLC）对MeOH级分进行纯化，得到了4-羟基苯甲酸（）、木犀草素（）和芹菜素（）作为其主要成分。这些化合物先前已被证明可降低TNF-α、IL-1β和IL-6的水平，是地上部分的一些主要生物活性成分。本研究是对的抗氧化和抗炎特性的首次研究，也是首次从该物种中分离出小分子。这些结果突出了利用海草治疗炎症的潜力以及进一步研究的必要性。
