Liang Yongqi, Weng Xinying, Ling Hao, Mustafa Ghazala, Yang Bingxian, Lu Na
Shanxi Qingmei Biotechnology Company Limited, Baoji 721000, China.
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310000, China.
Plants (Basel). 2024 Jun 7;13(12):1582. doi: 10.3390/plants13121582.
Lettuce is a widely consumed leafy vegetable; it became popular due to its enhanced nutritional content. Recently, lettuce is also regarded as one of the model plants for vegetable production in plant factories. Light and nutrients are essential environmental factors that affect lettuce growth and morphology. To evaluate the impact of light spectra on lettuce, butter lettuce was grown under the light wavelengths of 460, 525, and 660 nm, along with white light as the control. Plant morphology, physiology, nutritional content, and transcriptomic analyses were performed to study the light response mechanisms. The results showed that the leaf fresh weight and length/width were higher when grown at 460 nm and lower when grown at 525 nm compared to the control treatment. When exposed to 460 nm light, the sugar, crude fiber, mineral, and vitamin concentrations were favorably altered; however, these levels decreased when exposed to light with a wavelength of 525 nm. The transcriptomic analysis showed that co-factor and vitamin metabolism- and secondary metabolism-related genes were specifically induced by 460 nm light exposure. Furthermore, the pathway enrichment analysis found that flavonoid biosynthesis- and vitamin B6 metabolism-related genes were significantly upregulated in response to 460 nm light exposure. Additional experiments demonstrated that the vitamin B6 and B2 content was significantly higher in leaves exposed to 460 nm light than those grown under the other conditions. Our findings suggested that the addition of 460 nm light could improve lettuce's biomass and nutritional value and help us to further understand how the light spectrum can be tuned as needed for lettuce production.
生菜是一种广泛食用的叶菜类蔬菜；因其丰富的营养成分而受到欢迎。近年来，生菜也被视为植物工厂蔬菜生产的模式植物之一。光照和养分是影响生菜生长和形态的重要环境因素。为了评估光谱对生菜的影响，以奶油生菜为材料，在460、525和660nm的光波长下种植，并以白光作为对照。通过对植物形态、生理、营养成分和转录组分析来研究光响应机制。结果表明，与对照处理相比，在460nm光照下生长的叶片鲜重和长宽比更高，而在525nm光照下生长的则更低。当暴露于460nm光照下时，糖、粗纤维、矿物质和维生素的浓度发生了有利变化；然而，当暴露于525nm波长的光下时，这些水平下降。转录组分析表明，辅因子和维生素代谢以及次生代谢相关基因在460nm光照下被特异性诱导。此外，通路富集分析发现，类黄酮生物合成和维生素B6代谢相关基因在460nm光照下显著上调。进一步的实验表明，暴露于460nm光照下的叶片中维生素B6和B2的含量显著高于在其他条件下生长的叶片。我们的研究结果表明，添加460nm光照可以提高生菜的生物量和营养价值，并有助于我们进一步了解如何根据生菜生产的需要调整光谱。
