Torquetenovirus（TTV）在中重度 COVID-19 住院患者口腔脱落情况的调查。
Investigation of Oral Shedding of Torquetenovirus (TTV) in Moderate-to-Severe COVID-19 Hospitalised Patients.
机构信息
Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo 05508-000, Brazil.
Laboratory of Virology (LIM-52-HCFMUSP), Institute of Tropical Medicine, University of São Paulo School of Medicine, São Paulo 05403-000, Brazil.
出版信息
Viruses. 2024 May 24;16(6):831. doi: 10.3390/v16060831.
BACKGROUND
Torquetenovirus (TTV) is a small DNA virus constituting the human virome. High levels of TTV-DNA have been shown to be associated with immunosuppression and inflammatory chronic disorders.
AIM
To assess the possible association between the salivary viral load of TTV-DNA in patients hospitalised due to COVID-19 and disease severity.
METHODS
Saliva samples collected from 176 patients infected with SARS-CoV-2 were used to investigate the presence of SARS-CoV-2 and TTV-DNA by use of real-time RT-PCR.
RESULTS
The majority of patients were male with severe COVID-19. Presence of SARS-CoV-2 was observed in the saliva of 64.77% of patients, showing TTV-DNA in 55.68% of them. Patients with impaired clinical conditions ( < 0.001), which evolved to death ( = 0.003), showed a higher prevalence of TTV-DNA. The median viral load in patients with severe condition was 4.99 log copies/mL, in which those who were discharged and those evolving to death had values of 3.96 log copies/mL and 6.27 log copies/mL, respectively. A statistically significant association was found between the distribution of TTV-DNA viral load in saliva samples and severity of COVID-19 ( = 0.004) and disease outcomes ( < 0.001).
CONCLUSIONS
These results indicate that TTV-DNA in saliva could be a useful biomarker of COVID-19 severity and prognosis.
背景
Torquetenovirus（TTV）是一种构成人类病毒组的小型 DNA 病毒。高水平的 TTV-DNA 已被证明与免疫抑制和炎症性慢性疾病有关。
目的
评估因 COVID-19 住院的患者唾液中 TTV-DNA 的病毒载量与疾病严重程度之间的可能关联。
方法
使用实时 RT-PCR 检测 176 例感染 SARS-CoV-2 的患者唾液样本中 SARS-CoV-2 和 TTV-DNA 的存在情况。
结果
大多数患者为男性，患有严重的 COVID-19。在 64.77%的患者唾液中观察到 SARS-CoV-2 的存在，其中 55.68%的患者存在 TTV-DNA。临床状况受损的患者（<0.001），即发展为死亡的患者（=0.003），TTV-DNA 的患病率更高。严重病情患者的中位病毒载量为 4.99 log 拷贝/mL，其中出院患者和死亡患者的病毒载量分别为 3.96 log 拷贝/mL 和 6.27 log 拷贝/mL。在唾液样本中 TTV-DNA 病毒载量的分布与 COVID-19 的严重程度（=0.004）和疾病结局（<0.001）之间存在统计学显著关联。
结论
这些结果表明，唾液中的 TTV-DNA 可能是 COVID-19 严重程度和预后的有用生物标志物。
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