Academy for Engineering and Technology, Fudan University, China.
School of Information Science and Technology, Fudan University, Shanghai, China.
J Biophotonics. 2024 Aug;17(8):e202400071. doi: 10.1002/jbio.202400071. Epub 2024 Jun 27.
Photobiomodulation (PBM) using 460 nm blue light has been shown to have an inhibitory effect on skin cancer cells. In this study, we used a continuous LED light source with a wavelength of 460 nm and designed various combinations of power density (ranging from 6.4 to 25.6 mW) and dose (ranging from 0.96 to 30.72 J/cm) to conduct treatment experiments on MeWo cells to investigate the effects of blue light on MeWo melanoma cells. We are focusing on cell viability, cytotoxicity, mitochondrial function, oxidative stress, and apoptosis. We found that blue light inhibits these melanoma cells through oxidative stress and DNA damage, and this inhibition intensifies at higher irradiance levels. Although the cells initially attempt to resist the stress induced by the treatment, they eventually undergo apoptosis over time. These findings contribute to understanding melanoma's molecular response to blue light PBM, lay the groundwork for future clinical applications.
采用 460nm 蓝光的光生物调节（PBM）已被证明对皮肤癌细胞具有抑制作用。在这项研究中，我们使用了波长为 460nm 的连续 LED 光源，并设计了不同功率密度（范围为 6.4 至 25.6mW）和剂量（范围为 0.96 至 30.72J/cm）的组合对 MeWo 细胞进行治疗实验，以研究蓝光对 MeWo 黑色素瘤细胞的影响。我们专注于细胞活力、细胞毒性、线粒体功能、氧化应激和细胞凋亡。我们发现蓝光通过氧化应激和 DNA 损伤抑制这些黑色素瘤细胞，并且这种抑制在更高的辐照度水平下加剧。尽管细胞最初试图抵抗治疗引起的应激，但随着时间的推移，它们最终会发生凋亡。这些发现有助于了解黑色素瘤对蓝光 PBM 的分子反应，为未来的临床应用奠定基础。
