Ng T B, Wong C M, Li W W, Yeung H W
Comp Biochem Physiol B. 1985;81(2):503-4. doi: 10.1016/0305-0491(85)90350-5.
Lectins from the horseshoe crab (Limulus polyphemus) and the garden snail (Helix pomatia) were tested for insulinomimetic activities in isolated rat epididymal adipocytes. The sialic acid binding horseshoe crab lectin suppressed epinephrine-induced lipolysis and augmented lipogenesis from D-[3-3H]-glucose while the N-acetylgalactosamine binding snail lectin was inactive. The results suggest that the insulin receptor on rat adipocytes contains sialic acid in its carbohydrate moiety but does not possess non-reducing alpha-D-galactopyranosyl or 2-acetamido-2-deoxy-alpha-D-galactopyranosyl end groups.
检测了鲎（美洲鲎）和花园蜗牛（苹果蜗牛）的凝集素在分离的大鼠附睾脂肪细胞中的拟胰岛素活性。结合唾液酸的鲎凝集素抑制肾上腺素诱导的脂肪分解，并增强从D-[3-3H]-葡萄糖的脂肪生成，而结合N-乙酰半乳糖胺的蜗牛凝集素则无活性。结果表明，大鼠脂肪细胞上的胰岛素受体在其碳水化合物部分含有唾液酸，但不具有非还原性α-D-吡喃半乳糖基或2-乙酰氨基-2-脱氧-α-D-吡喃半乳糖基末端基团。
