Fedorov S N, Kalinovskiĭ V P, Serova O M, Seĭts I F
Eksp Onkol. 1985;7(3):31-4.
Application of preparative electrophoresis in agarose gel for isolation of chromosomal and extrachromosomal genetic elements is described. The above-mentioned method is used for fractionation of chromosomal DNA according to the molecular weight; for isolation of the plasmid containing the insertion of the viral oncogene myc and for separation of the viral oncogene myc insertion from the vector (a linear form of plasmid pBR 322). The method can be successfully applied in molecular biology, microbiology and medicine.
本文描述了制备性琼脂糖凝胶电泳在分离染色体和染色体外遗传元件中的应用。上述方法用于根据分子量对染色体DNA进行分级分离;用于分离含有病毒癌基因myc插入片段的质粒,以及用于从载体(质粒pBR 322的线性形式)中分离病毒癌基因myc插入片段。该方法可成功应用于分子生物学、微生物学和医学领域。