Wakabayashi S, Kagawa S, Nakao K, Matsuoka A
Nihon Naibunpi Gakkai Zasshi. 1985 May 20;61(5):592-604. doi: 10.1507/endocrine1927.61.5_592.
Monolayer islet cells prepared from neonatal rat pancreases were cultured in media with 5.5 mM glucose alone or supplemented with 1 mM 2-deoxy-2-fluoroglucose for a total of 7 days. The recovery of insulin in the cells and media, and the insulin secretory responses to four secretagogues were determined on several occasions. Under culture conditions with a basal medium containing 5.5 mM glucose, the responses to insulin secretagogues tested were abolished after 7 days of culture. In contrast, the addition of 2-deoxy-2-fluoroglucose maintained the insulin secretory responses to glucose (2.8 approximately 33.4 mM), leucine (2.5 approximately 10 mM) and 2-ketoisocaproate (2.5 approximately 10 mM) at levels several times higher than at day 0. Moreover, in these monolayer islet cells the rates of 0.62 mM [U-14C]glutamine oxidation were significantly increased by leucine or 2-ketoisocaproate at 10 mM. From these results, it is suggested that a series of metabolic adaptations in response to change in nutritional status (possibly attributable to the action of 2-deoxy-2-fluoroglucose as an inhibitor of glycolysis) may facilitate the metabolism of amino acids in B cells. Such an activation may be associated with the survival and maturation of neonatal B cells in vitro.
从新生大鼠胰腺制备的单层胰岛细胞在仅含5.5 mM葡萄糖或添加1 mM 2-脱氧-2-氟葡萄糖的培养基中培养7天。多次测定细胞和培养基中胰岛素的回收率以及对四种促分泌剂的胰岛素分泌反应。在含有5.5 mM葡萄糖的基础培养基的培养条件下，培养7天后对所测试的胰岛素促分泌剂的反应消失。相比之下，添加2-脱氧-2-氟葡萄糖可使对葡萄糖（2.8至33.4 mM）、亮氨酸（2.5至10 mM）和2-酮异己酸（2.5至10 mM）的胰岛素分泌反应维持在比第0天高出几倍的水平。此外，在这些单层胰岛细胞中，10 mM的亮氨酸或2-酮异己酸可使0.62 mM [U-14C]谷氨酰胺氧化速率显著增加。从这些结果表明，一系列响应营养状态变化的代谢适应（可能归因于2-脱氧-2-氟葡萄糖作为糖酵解抑制剂的作用）可能促进B细胞中氨基酸的代谢。这种激活可能与体外新生B细胞的存活和成熟有关。
