通过显微手术和免疫学方法消除蝗虫的促咽侧体活性：咽侧体、血淋巴蛋白和卵巢发育的体液控制证据

Elimination of the allatotropic activity in locusts by microsurgical and immunological methods: evidence for humoral control of the corpora allata, hemolymph proteins, and ovary development.

作者信息

Ulrich G M, Schlagintweit B, Eder J, Rembold H

出版信息

Gen Comp Endocrinol. 1985 Jul;59(1):120-9. doi: 10.1016/0016-6480(85)90426-5.

Abstract

Transection of the nervous connections (NCC I) between the brain and the corpus cardiacum (group 1) and injection of anti-brain antibodies inhibited vitellogenin synthesis and ovary development in virgin Locusta migratoria females. Removal of the neurosecretory material by these methods changed the titer and the electrophoretic pattern of hemolymph proteins, including vitellogenin. Transection of NCC I at some distance from the brain resulted in bulbs with 30-200 micron diameter which were filled with stainable neurosecretory material (group 2). Oocyte maturation was delayed in group 2 but normal eggs were laid. The same happened if group 1 animals received daily injections of JH-III. It is concluded that a humoral factor is involved in the activation of JH-III biosynthesis. The factor is transported through the NCC I and is released into the hemolymph as allatotropin.

摘要

切断大脑与心侧体之间的神经连接（NCC I，第1组）以及注射抗脑抗体，会抑制未交配的飞蝗雌性个体中卵黄原蛋白的合成和卵巢发育。通过这些方法去除神经分泌物质，会改变血淋巴蛋白（包括卵黄原蛋白）的滴度和电泳图谱。在距大脑一定距离处切断NCC I，会形成直径为30 - 200微米的球状物，其中充满了可染色的神经分泌物质（第2组）。第2组的卵母细胞成熟延迟，但仍能产下正常的卵。如果给第1组动物每日注射保幼激素III（JH - III），也会出现同样的情况。由此得出结论，一种体液因子参与了JH - III生物合成的激活过程。该因子通过NCC I运输，并作为促咽侧体素释放到血淋巴中。