Department of Environmental Engineering and Research Center for Environmental and Hazardous Substance Management, Faculty of Engineering, Khon Kaen University, 40002, Thailand.
Department of Environmental Engineering and Research Center for Environmental and Hazardous Substance Management, Faculty of Engineering, Khon Kaen University, 40002, Thailand.
Environ Pollut. 2024 Sep 15;357:124456. doi: 10.1016/j.envpol.2024.124456. Epub 2024 Jun 26.
Triclocarban (TCC), an antibacterial agent commonly used in personal care products, is one of the top ten contaminants of emerging concern in various environmental media, including soil and contaminated water in vadose zone. This study aimed to investigate TCC-contaminated water remediation using biochar-immobilized bacterial cells. Pseudomonas fluorescens strain MC46 (MC46), an efficient TCC-degrading isolate, was chosen, whereas agro-industrial carbonized waste as biochar was directly used as a sustainable cell immobilization carrier. According to the long-term TCC removal performance results (160 d), the biochar-immobilized cells consistently exhibited high TCC removal efficiencies (84-97%), whereas the free MC46 removed TCC for 76-94%. At 100 days, the detachment of the MC46 cells from the immobilized cell column was observed. The micro-Fourier-transform infrared spectroscopy results indicated that extracellular polymeric substance (EPS) was produced, but polysaccharide and protein fractions were washed out of the column. The lipid fraction of EPS adhered to the biochar, promoting TCC sorption for long-term treatment. The shortening of MC46 cells improved the tolerance of TCC toxicity. The TCC-contaminated water was successfully detoxified by the biochar-immobilized MC46 cells. Overall, the waste-derived biochar-immobilized cell system proposed in this study for the removal of emerging contaminants, including TCC, is efficient, economical, and aligned with the sustainable development concept of value-added utilization of waste.
三氯卡班（TCC）是一种常用的抗菌剂，广泛应用于个人护理产品中，它是包括土壤和包气带污染水中在内的各种环境介质中十大新兴关注污染物之一。本研究旨在利用生物炭固定化细菌细胞来修复 TCC 污染的水。选择了荧光假单胞菌（MC46）作为一种高效的 TCC 降解菌，而农业工业碳化废物则直接用作可持续的细胞固定化载体。根据长期 TCC 去除性能结果（160 天），生物炭固定化细胞始终表现出较高的 TCC 去除效率（84-97%），而游离的 MC46 则去除了 76-94%的 TCC。在 100 天时，观察到 MC46 细胞从固定化细胞柱上脱落。微傅里叶变换红外光谱结果表明，细胞外聚合物（EPS）被产生，但多糖和蛋白质部分从柱中被冲洗出来。EPS 的脂质部分附着在生物炭上，促进了 TCC 的长期吸附。MC46 细胞的缩短提高了其对 TCC 毒性的耐受性。TCC 污染的水被生物炭固定化 MC46 细胞成功解毒。总体而言，本研究提出的利用废物衍生的生物炭固定化细胞系统来去除新兴污染物，包括 TCC，是高效、经济的，符合废物增值利用的可持续发展理念。
