Hirose M, Sarui K, Sunagawa A
J Biochem. 1985 Mar;97(3):781-9. doi: 10.1093/oxfordjournals.jbchem.a135118.
Sodium butyrate prevented the accumulation of casein mRNA induced by the combined action of prolactin and glucocorticoid in the presence of insulin in the cultured mammary gland. This inhibition was reversible and dose-dependent. In addition to the inhibition of the mRNA induction, both nuclear histone acetylase and deacetylase activities were inhibited by the incubation of the glands with butyrate, whereas these enzyme activities were stimulated by glucocorticoid and prolactin, or by glucocorticoid alone, in the presence of insulin. These data strongly suggest that the increased metabolism of histone acetyl groups is involved in the hormone-mediated casein mRNA induction and that glucocorticoid plays a preferential role on this increased metabolism.
丁酸钠可防止在培养的乳腺中，胰岛素存在的情况下，催乳素和糖皮质激素联合作用所诱导的酪蛋白mRNA的积累。这种抑制作用是可逆的且呈剂量依赖性。除了抑制mRNA的诱导外，用丁酸盐孵育腺体还会抑制核组蛋白乙酰化酶和去乙酰化酶的活性，而在胰岛素存在的情况下，糖皮质激素和催乳素联合作用或单独使用糖皮质激素会刺激这些酶的活性。这些数据有力地表明，组蛋白乙酰基代谢的增加参与了激素介导的酪蛋白mRNA诱导，并且糖皮质激素在这种增加的代谢中起优先作用。
