Dr Mahabuba Azmary Hossain, M Phil (Pharmacology), Final Part Student, Department of Pharmacology, Mymensingh Medical College (MMC), Mymensingh, Bangladesh; E-mail:
Mymensingh Med J. 2024 Jul;33(3):649-655.
Antibiotics' usefulness is threatened by multi-drugs resistance in harmful microorganisms because of abuse and regulatory problems. Emerging microbes, resistance mechanisms and antimicrobial drugs all require extensive investigation. Evaluation of the in vitro antibacterial activity of Methanolic extracts isolated from Black pepper seeds (Piper nigrum L.) against two infection causing pathogens, Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. From July 2022 and June 2023, this experimental study was conducted at the Mymensingh Medical College's Department of Pharmacology and Therapeutics in conjunction with the Department of Microbiology. The solvents Methanol and 10.0% Di-Methyl Sulfoxide (DMSO) were used to make the extract. Using the disc diffusion and broth dilution methods, the antibacterial activity of methanolic extract of black pepper seeds (MBPE) was evaluated at various doses. Using the broth dilution procedure, the conventional antibiotic Ciprofloxacin was utilized, and the outcome was contrasted with that of Methanol extracts. Methanolic extract of black pepper seeds (MBPE) at seven distinct concentrations (100, 80, 60, 40, 20, 10 and 5mg/ml) were utilized, then later in chosen concentrations as needed to confirm the extracts' more precise margin of antimicrobial sensitivity. At 80mg/ml and above doses of the MBPE, it had an inhibitory impact against the aforementioned microorganisms. For Staphylococcus aureus and Pseudomonas aeruginosa the MIC were 60 and 70mg/ml in MBPE respectively. As of the MIC of Ciprofloxacin was 1μg/ml against Staphylococcus aureus and 1.5μg/ml for Pseudomonas aeruginosa. In comparison to MICs of MBPE for the test organisms, the MIC of Ciprofloxacin was the lowest. This study clearly shows that Staphylococcus aureus and Pseudomonas aeruginosa are sensitive to the methanolic extract of black pepper seeds' antibacterial properties.
抗生素的滥用和监管问题导致有害微生物出现了多药耐药性，从而使其功效受到了威胁。新兴微生物、耐药机制和抗菌药物都需要广泛的研究。评估从黑胡椒种子（Piper nigrum L.）中分离得到的甲醇提取物对两种感染病原体，革兰氏阳性金黄色葡萄球菌和革兰氏阴性铜绿假单胞菌的体外抗菌活性。本实验研究于 2022 年 7 月至 2023 年 6 月在孟加拉国迈门辛医科大学药理学与治疗学系与微生物学系合作进行。使用甲醇和 10.0%二甲基亚砜（DMSO）作为溶剂制备提取物。采用纸片扩散法和肉汤稀释法，在不同剂量下评价黑胡椒种子甲醇提取物（MBPE）的抗菌活性。采用肉汤稀释法，使用常规抗生素环丙沙星作为对照，并与甲醇提取物的结果进行比较。利用七种不同浓度（100、80、60、40、20、10 和 5mg/ml）的黑胡椒种子甲醇提取物（MBPE），然后根据需要在选定浓度下进一步确认提取物更精确的抗菌敏感性范围。MBPE 在 80mg/ml 及以上剂量时，对上述两种微生物均有抑制作用。MBPE 对金黄色葡萄球菌和铜绿假单胞菌的 MIC 分别为 60 和 70mg/ml。而环丙沙星对金黄色葡萄球菌和铜绿假单胞菌的 MIC 分别为 1μg/ml 和 1.5μg/ml。与 MBPE 对测试生物的 MIC 相比，环丙沙星的 MIC 最低。本研究清楚地表明，金黄色葡萄球菌和铜绿假单胞菌对黑胡椒种子甲醇提取物的抗菌特性敏感。
