State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; State Environmental Protection Key Laboratory of Ecological Effect and Risk Assessment of Chemicals, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; State Environmental Protection Key Laboratory of Ecological Effect and Risk Assessment of Chemicals, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
Bioresour Technol. 2024 Aug;406:131048. doi: 10.1016/j.biortech.2024.131048. Epub 2024 Jun 28.
The nitrogen loss in composting is primarily driven by the transformation of organic nitrogen, yet the mechanisms underlying the degradation process remain incompletely understood. This study employed protein family domains (Pfams) analysis based on metagenomic sequencing to investigate the functional characteristics, key microorganisms, and environmental parameters influencing organic nitrogen degradation in chicken manure and pig manure composting. 154 Pfams associated with ammonification function were identified. Predominant Pfams: proteolytic peptidase, followed by chitin/cell wall degraders, least involved in nucleic acid degradation. Ammonifying microbial diversity was basically consistent among compost types, particularly in the thermophilic stage with the peak of abundance of dominant ammonifying microorganisms. Viruses played an important role in ammonification process, especially Uroviricota. 26 key ammonifying genera were identified by the microbial network. pH dominated the metabolic activity of ammonifying microorganisms in various manure compost types, primarily consisting of protein-degrading bacteria with stable community structures.
堆肥过程中的氮损失主要是由有机氮的转化驱动的，但降解过程的机制仍不完全清楚。本研究采用基于宏基因组测序的蛋白质家族域（Pfams）分析，研究了鸡粪和猪粪堆肥中有机氮降解的功能特征、关键微生物和环境参数。鉴定出与氨化功能相关的 154 个 Pfams。主要 Pfams：蛋白水解肽酶，其次是壳聚糖/细胞壁降解酶，核酸降解参与最少。氨化微生物多样性在堆肥类型中基本一致，特别是在高温阶段，优势氨化微生物的丰度达到峰值。病毒在氨化过程中起着重要作用，特别是 Uroviricota。通过微生物网络鉴定出 26 个关键的氨化属。pH 主导各种粪肥堆肥类型中氨化微生物的代谢活性，主要由具有稳定群落结构的蛋白降解细菌组成。
