使用埃及血吸虫可溶性虫卵粗抗原的酶联免疫吸附测定法与使用纯化曼氏血吸虫虫卵抗原的放射免疫测定法在埃及血吸虫病血清诊断中的比较。

Comparison of an enzyme-linked immunosorbent assay using crude S. haematobium soluble egg antigen with a radioimmunoassay using purified S. mansoni egg antigen for the serodiagnosis of schistosomiasis haematobium.

作者信息

Stek M, Kassim O O, Hansen K, Pelley R P

出版信息

J Trop Med Hyg. 1985 Feb;88(1):1-6.

Abstract

An enzyme-linked immunosorbent assay (ELISA) using crude Schistosoma haematobium soluble egg antigen (ShSEA) was compared with a radioimmunoassay (RIA) employing purified heterologous species S. mansoni egg antigen (MSA1) for the serodiagnosis of schistosomiasis haematobium in a group of 45 Nigerian school children living in an area endemic for S. haematobium. Both assay systems appeared applicable. The ELISA proved to be more sensitive detecting 100% of the 27 parasitologically positive individuals while the RIA defined 89% of this group. Neither test had false-positive results for the 10 non-endemic area parasitologically negative controls, although the ELISA demonstrated that 56% of the 18 endemic area parasitologically negative controls had anti-ShSEA antibodies. The RIA indicated that 44% of this group had anti-MSA1 antibodies. These latter findings were interpreted as related to the hyperendemicity of schistosomiasis haematobium for the study area.

摘要

在一组45名生活在埃及血吸虫病流行地区的尼日利亚学童中，将使用埃及血吸虫可溶性虫卵粗抗原（ShSEA）的酶联免疫吸附测定（ELISA）与采用纯化的异源物种曼氏血吸虫虫卵抗原（MSA1）的放射免疫测定（RIA）用于埃及血吸虫病的血清学诊断进行比较。两种检测系统似乎都适用。ELISA检测更为灵敏，在27名寄生虫学检测呈阳性的个体中检测出100%，而RIA检测出该组中的89%。对于10名非流行地区寄生虫学检测呈阴性的对照，两种检测均无假阳性结果，不过ELISA显示，18名流行地区寄生虫学检测呈阴性的对照中有56%具有抗ShSEA抗体。RIA表明该组中有44%具有抗MSA1抗体。后一项发现被解释为与研究地区埃及血吸虫病的高度流行有关。