Stek M, Kassim O O, Hansen K, Pelley R P
J Trop Med Hyg. 1985 Feb;88(1):1-6.
An enzyme-linked immunosorbent assay (ELISA) using crude Schistosoma haematobium soluble egg antigen (ShSEA) was compared with a radioimmunoassay (RIA) employing purified heterologous species S. mansoni egg antigen (MSA1) for the serodiagnosis of schistosomiasis haematobium in a group of 45 Nigerian school children living in an area endemic for S. haematobium. Both assay systems appeared applicable. The ELISA proved to be more sensitive detecting 100% of the 27 parasitologically positive individuals while the RIA defined 89% of this group. Neither test had false-positive results for the 10 non-endemic area parasitologically negative controls, although the ELISA demonstrated that 56% of the 18 endemic area parasitologically negative controls had anti-ShSEA antibodies. The RIA indicated that 44% of this group had anti-MSA1 antibodies. These latter findings were interpreted as related to the hyperendemicity of schistosomiasis haematobium for the study area.
在一组45名生活在埃及血吸虫病流行地区的尼日利亚学童中，将使用埃及血吸虫可溶性虫卵粗抗原（ShSEA）的酶联免疫吸附测定（ELISA）与采用纯化的异源物种曼氏血吸虫虫卵抗原（MSA1）的放射免疫测定（RIA）用于埃及血吸虫病的血清学诊断进行比较。两种检测系统似乎都适用。ELISA检测更为灵敏，在27名寄生虫学检测呈阳性的个体中检测出100%，而RIA检测出该组中的89%。对于10名非流行地区寄生虫学检测呈阴性的对照，两种检测均无假阳性结果，不过ELISA显示，18名流行地区寄生虫学检测呈阴性的对照中有56%具有抗ShSEA抗体。RIA表明该组中有44%具有抗MSA1抗体。后一项发现被解释为与研究地区埃及血吸虫病的高度流行有关。
