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酶联免疫吸附测定法在诊断中的应用。

The enzyme linked immunosorbent assay in the diagnosis.

作者信息

Farag H F, Barakat R M

出版信息

Tropenmed Parasitol. 1978 Mar;29(1):12-4.

PMID:644655
Abstract

This study included 72 persons with ova of S. mansoni and/or S. haematobium in their stool or urine, 16 persons from Schistosomiasis-endemic areas but without demonstration of ova, and 9 healthy persons from areas with no schistosomiasis who served as controls. The sera were examined with ELISA using antigen from adult S. mansoni worms. All parasitologically proven cases had extinction values higher than those of controls. False positive results were obtained in 11 out of 16 persons from endemic areas but without ova. These results may have been due to cross-reactions, a past history of schistosomiasis or a missed diagnosis of S. mansoni-infections. Cases with S. haematobium and cases with S. mansoni showed signifcantly different extinction values. The differences between both sexes were not statistically significant.

摘要

本研究纳入了72名粪便或尿液中存在曼氏血吸虫和/或埃及血吸虫虫卵的人、16名来自血吸虫病流行区但未检出虫卵的人,以及9名来自无血吸虫病地区的健康人作为对照。采用曼氏血吸虫成虫抗原的ELISA法检测血清。所有经寄生虫学证实的病例的吸光度值均高于对照组。在16名来自流行区但未检出虫卵的人中,有11人出现假阳性结果。这些结果可能是由于交叉反应、既往血吸虫病史或曼氏血吸虫感染漏诊所致。埃及血吸虫病例和曼氏血吸虫病例的吸光度值存在显著差异。两性之间的差异无统计学意义。

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