多位点序列分型（MLST）与转录组的相互作用揭示中国贵阳分离的碳青霉烯耐药临床菌株的抗生素耐药机制
Cross-talk of MLST and transcriptome unveiling antibiotic resistance mechanism of carbapenem resistance clinical strains isolated in Guiyang, China.
作者信息
Qiu Zhilang, Yuan Kexin, Cao Huijun, Chen Sufang, Chen Feifei, Mo Fei, Guo Guo, Peng Jian
机构信息
Center for Clinical Laboratories, The Affiliated Hospital of Guizhou Medical University, Guiyang, China.
Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Cellular Immunotherapy Engineering Research Center of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China.
出版信息
Front Microbiol. 2024 Jun 14;15:1394775. doi: 10.3389/fmicb.2024.1394775. eCollection 2024.
INTRODUCTION
() is an important opportunistic pathogen causing nosocomial infection in the clinic. The occurrence rate of antibiotic resistance is increasing year by year, resulting in a highly serious situation of bacterial resistance.
METHODS
To better understand the local epidemiology of multidrug-resistant , an investigation was conducted on the antibiotic resistance of different types of and its relationship with the genes of . Furthermore, the molecular mechanism underlying antibiotic resistance in was investigated through transcriptome analysis.
RESULTS
These results showed that a total of 9 STs were detected. It was found that 99% of the strains isolated in the hospital belonged to the same STs, and the clone complex CC208 was widely distributed in various departments and all kinds of samples. Furthermore, these strains showed high resistance to ertapenem, biapenem, meropenem, and imipenem, among which the resistance to ertapenem was the strongest. The detection rate of gene in these carbapenem resistance (CRAB) reached 100%; Additionally, the transcriptome results showed that the resistance genes were up-regulated in resistance strains, and these genes involved in biofilm formation, efflux pumps, peptidoglycan biosynthesis, and chaperonin synthesis.
DISCUSSION
These results suggest that the CC208 STs were the main clonal complex, and showed high carbapenem antibiotic resistance. All these resistant strains were distributed in various departments, but most of them were distributed in intensive care units (ICU). The was the main antibiotic resistance genotype; In summary, the epidemic trend of clinical in Guiyang, China was analyzed from the molecular level, and the resistance mechanism of to carbapenem antibiotics was analyzed with transcriptome, which provided a theoretical basis for better control of .
引言
（某菌）是临床上引起医院感染的重要机会致病菌。抗生素耐药率逐年上升，导致细菌耐药形势极为严峻。
方法
为更好地了解多重耐药（某菌）的当地流行病学情况，对不同类型（某菌）的抗生素耐药性及其与（某菌）基因的关系进行了调查。此外，通过转录组分析研究了（某菌）抗生素耐药的分子机制。
结果
这些结果表明共检测到9种序列类型（STs）。发现医院分离的菌株中有99%属于同一STs，克隆复合体CC208广泛分布于各个科室和各类样本中。此外，这些（某菌）菌株对厄他培南、比阿培南、美罗培南和亚胺培南表现出高度耐药，其中对厄他培南的耐药性最强。这些耐碳青霉烯类（某菌）（CRAB）中（某基因）的检出率达到100%；此外，转录组结果显示耐药菌株中耐药基因上调，这些基因参与生物膜形成、外排泵、肽聚糖生物合成和伴侣蛋白合成。
讨论
这些结果表明CC208 STs是主要的克隆复合体，且表现出高度的碳青霉烯类抗生素耐药性。所有这些耐药菌株分布于各个科室，但大多数分布在重症监护病房（ICU）。（某基因）是主要的抗生素耐药基因型；总之，从分子水平分析了中国贵阳临床（某菌）的流行趋势，并用转录组分析了（某菌）对碳青霉烯类抗生素的耐药机制，为更好地控制（某菌）提供了理论依据。
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