利用商业 PCR 平台从鼻腔拭子中诊断毛霉菌病；一种可行的替代方法？
Diagnosis of mucormycosis from nasal swabs using commercial PCR platforms; a feasible alternative?
机构信息
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Department of Medicine, All India Institute of Medical Sciences, New Delhi, 110029, India.
出版信息
Indian J Med Microbiol. 2024 Jul-Aug;50:100661. doi: 10.1016/j.ijmmb.2024.100661. Epub 2024 Jul 2.
Rhino-orbital-cerebral mucormycosis (ROCM) is linked to uncontrolled diabetes, diabetic ketoacidosis, iron overload, corticosteroid therapy, and neutropenia. This study evaluated a commercial real-time PCR system's effectiveness in detecting Mucorales from nasal swabs in 50 high-risk patients. Nasal swab PCR showed 30% positivity, compared to 8% with KOH microscopy. Despite its improved sensitivity, nasal swab PCR has limitations, highlighting the importance of established sampling methods in mucormycosis diagnosis. Participants were predominantly male (64%), with diabetes (78%) and amphotericin B use (96%). Prior COVID-19 was 42%, with 30% positive for Mucorales by PCR, compared to 8% with KOH microscopy.
鼻脑型毛霉病（ROCM）与未控制的糖尿病、糖尿病酮症酸中毒、铁过载、皮质类固醇治疗和中性粒细胞减少症有关。本研究评估了一种商业实时 PCR 系统在检测 50 名高危患者鼻拭子中毛霉科的有效性。鼻拭子 PCR 的阳性率为 30%，而 KOH 显微镜检查为 8%。尽管其敏感性有所提高，但鼻拭子 PCR 仍有其局限性，这突出了在毛霉病诊断中建立采样方法的重要性。参与者主要为男性（64%），患有糖尿病（78%）和两性霉素 B 治疗（96%）。既往有 COVID-19 病史者占 42%，PCR 检测毛霉科阳性率为 30%，而 KOH 显微镜检查为 8%。
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