自闭症谱系障碍中瘦素水平的改变和脂肪因子的荟萃分析。

Altered leptin level in autism spectrum disorder and meta-analysis of adipokines.

机构信息

Key Laboratory of Ethnomedicine of Ministry of Education, Center On Translational Neuroscience, School of Pharmacy, Minzu University of China, 27 South Zhongguancun Avenue, Beijing, 100081, China.

Institute of National Security, Minzu University of China, Beijing, China.

出版信息

BMC Psychiatry. 2024 Jul 1;24(1):479. doi: 10.1186/s12888-024-05936-4.

DOI:10.1186/s12888-024-05936-4

PMID:38951775

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11218410/

Abstract

BACKGROUND

Increasing evidence suggests that leptin is involved in the pathology of autism spectrum disorder (ASD). In this study, our objective was to investigate the levels of leptin in the blood of children with ASD and to examine the overall profile of adipokine markers in ASD through meta-analysis.

METHODS

Leptin concentrations were measured using an enzyme-linked immunosorbent assay (ELISA) kit, while adipokine profiling, including leptin, was performed via meta-analysis. Original reports that included measurements of peripheral adipokines in ASD patients and healthy controls (HCs) were collected from databases such as Web of Science, PubMed, and Cochrane Library. These studies were collected from September 2022 to September 2023 and followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Standardized mean differences were calculated using a random effects model for the meta-analysis. Additionally, we performed meta-regression and explored heterogeneity among studies.

RESULTS

Our findings revealed a significant increase in leptin levels in children with ASD compared to HCs (p = 0.0319). This result was consistent with the findings obtained from the meta-analysis (p < 0.001). Furthermore, progranulin concentrations were significantly reduced in children with ASD. However, for the other five adipokines analyzed, there were no significant differences observed between the children with ASD and HCs children. Heterogeneity was found among the studies, and the meta-regression analysis indicated that publication year and latitude might influence the results of the meta-analysis.

CONCLUSIONS

These findings provide compelling evidence that leptin levels are increased in children with ASD compared to healthy controls, suggesting a potential mechanism involving adipokines, particularly leptin, in the pathogenesis of ASD. These results contribute to a better understanding of the pathology of ASD and provide new insights for future investigations.

摘要

背景

越来越多的证据表明，瘦素参与了自闭症谱系障碍（ASD）的病理过程。在这项研究中，我们的目的是检测 ASD 患儿血液中的瘦素水平，并通过荟萃分析研究 ASD 患者的整体脂肪因子标志物谱。

方法

使用酶联免疫吸附测定（ELISA）试剂盒测量瘦素浓度，通过荟萃分析进行脂肪因子分析，包括瘦素。从 Web of Science、PubMed 和 Cochrane Library 等数据库中收集了包含 ASD 患者和健康对照（HC）外周脂肪因子测量的原始报告。这些研究于 2022 年 9 月至 2023 年 9 月收集，符合系统评价和荟萃分析的首选报告项目（PRISMA）指南。荟萃分析采用随机效应模型计算标准化均数差。此外，我们进行了荟萃回归分析，并探讨了研究之间的异质性。

结果

我们的研究结果显示，与 HCs 相比，ASD 患儿的瘦素水平显著升高（p=0.0319）。这一结果与荟萃分析的结果一致（p<0.001）。此外，ASD 患儿的颗粒蛋白聚糖浓度显著降低。然而，对于其他五种分析的脂肪因子，ASD 患儿与 HCs 之间没有观察到显著差异。研究之间存在异质性，荟萃回归分析表明，发表年份和纬度可能会影响荟萃分析的结果。