Department of Pediatrics, Shandong Provincial Third Hospital, Shandong University, No.11 Wuyingshan, Middle Road, Tianqiao District, Jinan, Shandong 250031, P.R. China.
J Microbiol Biotechnol. 2024 Jul 28;34(7):1419-1424. doi: 10.4014/jmb.2401.01018. Epub 2024 Apr 24.
Secretin PilQ is an antigenically conserved outer membrane protein that is present in most meningococci and PorA is a major protein that elicits bactericidal immune response in humans following natural disease and immunization. In the present study, BALB/c mice were immunized subcutaneously with rPilQ406-770 or rPorA together with Freund's adjuvant (FA). Serum antibody responses to serogroup A and B whole cells or purified proteins and functional activity of antibodies were determined by ELISA and serum bactericidal assay (SBA), respectively. Serum IgG responses were significantly increased in the immunized group with rPilQ406-770 or rPorA together with FA compared to control groups. IgG antibody response of mice immunized with rPilQ406-770 was significantly more than mice immunized with rPorA (OD at 450 nm was 1.6 versus 0.83). The booster injections were effective in increasing the responses of anti-rPilQ406-770 or anti-rPorA IgG significantly. Antisera produced against rPilQ406-770 or rPorA demonstrated strong surface reactivity to serogroup B in comparison with control groups. Antisera raised against rPorA or rPilQ406-770 and FA demonstrated SBA titers from 1/1024 to 1/2048 against serogroup B. The strongest bactericidal activity was detected in sera from mice immunized with rPilQ406-770 mixed with FA. These results suggest that rPilQ406-770 is a potential vaccine candidate for serogroup B N. .
分泌蛋白 PilQ 是一种抗原保守的外膜蛋白，存在于大多数脑膜炎奈瑟菌中，PorA 是一种主要蛋白，在自然感染和免疫接种后，能在人体中引发杀菌免疫反应。在本研究中，BALB/c 小鼠经皮下免疫 rPilQ406-770 或 rPorA 与福氏佐剂（FA）。通过 ELISA 和血清杀菌试验（SBA）分别测定针对血清 A 群和 B 群全细胞或纯化蛋白的血清抗体反应和抗体的功能活性。与对照组相比，rPilQ406-770 或 rPorA 与 FA 联合免疫组的血清 IgG 反应显著增加。rPilQ406-770 免疫小鼠的 IgG 抗体反应明显高于 rPorA 免疫小鼠（OD450nm 为 1.6 比 0.83）。加强注射能有效提高抗 rPilQ406-770 或抗 rPorA IgG 的反应。与对照组相比，针对 rPilQ406-770 或 rPorA 的抗血清显示出对血清 B 群更强的表面反应性。针对 rPorA 或 rPilQ406-770 和 FA 的抗血清对血清 B 群的 SBA 滴度为 1/1024 至 1/2048。在与 FA 混合免疫 rPilQ406-770 的小鼠血清中检测到最强的杀菌活性。这些结果表明 rPilQ406-770 是 B 群 N. 的潜在疫苗候选物。
