College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, 830054, China.
Mikrochim Acta. 2024 Jul 3;191(8):444. doi: 10.1007/s00604-024-06522-x.
Transferrin (TRF), recognized as a glycoprotein clinical biomarker and therapeutic target, has its concentration applicable for disease diagnosis and treatment monitoring. Consequently, this study developed boronic acid affinity magnetic surface molecularly imprinted polymers (B-MMIPs) with pH-responsitivity as the "capture probe" for TRF, which have high affinity similar to antibodies, with a dissociation constant of (3.82 ± 0.24) × 10 M, showing 7 times of reusability. The self-copolymerized imprinted layer synthesized with dopamine (DA) and 3-Aminophenylboronic acid (APBA) as double monomers avoided nonspecific binding sites and produced excellent adsorption properties. Taking the gold nanostar (AuNS) with a branch tip "hot spot" structure as the core, the silver-coated AuNS functionalized with the biorecognition element 4-mercaptophenylboronic acid (MPBA) was employed as a surface-enhanced Raman scattering (SERS) nanotag (AuNS@Ag-MPBA) to label TRF, thereby constructing a double boronic acid affinity "sandwich" SERS biosensor (B-MMIPs-TRF-SERS nanotag) for the highly sensitive detection of TRF. The SERS biosensor exhibited a detection limit for TRF of 0.004 ng/mL, and its application to spiked serum samples confirmed its reliability and feasibility, demonstrating significant potential for clinical TRF detection. Moreover, the SERS biosensor designed in this study offers advantages in stability, detection speed (40 min), and cost efficiency. The portable Raman instrument for SERS detection fulfills the requirements for point-of-care testing.
转铁蛋白（TRF）作为一种糖基化蛋白临床生物标志物和治疗靶点，其浓度适用于疾病诊断和治疗监测。因此，本研究开发了具有 pH 响应性的硼酸亲和磁性表面分子印迹聚合物（B-MMIPs）作为 TRF 的“捕获探针”，其具有与抗体相似的高亲和力，解离常数为（3.82±0.24）×10 -7 M，具有 7 倍的可重复使用性。以多巴胺（DA）和 3-氨基苯硼酸（APBA）为双单体自聚合印迹层，避免了非特异性结合位点，产生了优异的吸附性能。以具有分支尖端“热点”结构的金纳米星（AuNS）为核心，将生物识别元件 4-巯基苯硼酸（MPBA）功能化的银包覆 AuNS 作为表面增强拉曼散射（SERS）纳米标签（AuNS@Ag-MPBA）用于标记 TRF，从而构建了双硼酸亲和“三明治”SERS 生物传感器（B-MMIPs-TRF-SERS 纳米标签），用于 TRF 的高灵敏度检测。SERS 生物传感器对 TRF 的检测限为 0.004 ng/mL，对加标血清样品的应用证实了其可靠性和可行性，为临床 TRF 检测提供了重要的应用前景。此外，本研究设计的 SERS 生物传感器在稳定性、检测速度（40 分钟）和成本效益方面具有优势。用于 SERS 检测的便携式拉曼仪器满足即时检测的要求。
