Veterinary Science Doctoral Program, Faculty of Veterinary Medicine, Airlangga University, Kampus C Mulyorejo, Surabaya, East Java, Indonesia.
Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency, Cibinong Science Center, Bogor West Jawa Province, Indonesia.
Rev Bras Parasitol Vet. 2024 Jun 28;33(2):e001324. doi: 10.1590/S1984-29612024033. eCollection 2024.
Trypanosoma evansi is reportedly divided into two genotypes: types A and B. The type B is uncommon and reportedly limited to Africa: Kenya Sudan, and Ethiopia. In contrast, type A has been widely reported in Africa, South America, and Asia. However, Trypanosoma evansi type non-A/B has never been reported. Therefore, this study aims to determine the species and genotype of the Trypanozoon subgenus using a robust identification algorithm. Forty-three trypanosoma isolates from Indonesia were identified as Trypanosoma evansi using a molecular identification algorithm. Further identification showed that 39 isolates were type A and 4 isolates were possibly non-A/B types. The PML, AMN-SB1, and STENT3 isolates were likely non-A/B type Trypanosoma evansi isolated from buffalo, while the PDE isolates were isolated from cattle. Cladistic analysis revealed that Indonesian Trypanosoma evansi was divided into seven clusters based on the gRNA-kDNA minicircle gene. Clusters 6 and 7 are each divided into two sub-clusters. The areas with the highest genetic diversity are the provinces of Banten, Central Java (included Yogyakarta), and East Nusa Tenggara. The Central Java (including Yogyakarta) and East Nusa Tenggara provinces, each have four sub-clusters, while Banten has three.
A 型和 B 型。B 型较为罕见，据报道仅局限于非洲：肯尼亚、苏丹和埃塞俄比亚。相比之下，A 型已在非洲、南美洲和亚洲广泛报道。然而，从未报道过非 A/B 型伊氏锥虫。因此，本研究旨在使用强大的鉴定算法确定锥虫亚属的物种和基因型。使用分子鉴定算法从印度尼西亚鉴定出的 43 株锥虫分离株被鉴定为伊氏锥虫。进一步鉴定表明，39 株为 A 型，4 株可能为非 A/B 型。PML、AMN-SB1 和 STENT3 分离株可能是非 A/B 型伊氏锥虫，从水牛中分离，而 PDE 分离株从牛中分离。系统发育分析显示，印度尼西亚伊氏锥虫根据 gRNA-kDNA 微环基因分为七个聚类。第 6 和 7 个聚类各自分为两个亚聚类。遗传多样性最高的地区是万丹省、中爪哇省（包括日惹）和东努沙登加拉省。中爪哇省（包括日惹）和东努沙登加拉省各有四个亚聚类，而万丹省有三个。
