Department of Medicine (Neurology and Rheumatology), Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
Immunol Res. 2024 Oct;72(5):1108-1119. doi: 10.1007/s12026-024-09512-6. Epub 2024 Jul 3.
We investigated the phenotypic characteristics of human leukocyte antigen (HLA)-E-expressing macrophages, NKG2A/CD94 expression in T and natural killer (NK) cells, and their interactions in patients with adult-onset Still's disease (AOSD). Peripheral blood mononuclear cells from 22 patients with AOSD and 22 healthy controls (HC) were used. Isolated monocytes were cultured first with macrophage colony-stimulating factor to differentiate into M0 macrophages and subsequently with lipopolysaccharide/interferon-γ or interleukin-4 to differentiate into M1 or M2 macrophages, respectively. HLA-E and NKG2A/CD94 expression levels were evaluated using quantitative RT-PCR and flow cytometry. HLA-E expression in M0 and M2 macrophages was significantly higher in patients with AOSD than in HC, and was positively correlated with serum C-reactive protein levels and erythrocyte sedimentation rate. NKG2A/CD94 expression in CD4 + and CD8 + T cells was significantly higher in patients with AOSD than in HC, but that in NK cells was not significantly different. In patients with AOSD, NKG2A expression in CD4 + T cells positively correlated with HLA-E expression in M0, M1, and M2 macrophages. CD94 expression in CD8 + T cells inversely correlated with HLA-E expression in M1 and M2 macrophages. NKG2A and CD94 expression in NK cells inversely correlated with HLA-E expression in M0, M1, and M2 macrophages. No significant correlation was observed between HLA-E and NKG2A/CD94 expression in HC. Increased expression of HLA-E in macrophages and NKG2A/CD94 in T cells can be observed in the inflammatory condition of AOSD. HLA-E-expressing macrophages may be associated with NKG2A/CD94 expression in T and NK cells with different correlations.
我们研究了人白细胞抗原（HLA）-E 表达的巨噬细胞、T 和自然杀伤（NK）细胞中的 NKG2A/CD94 表达及其在成年Still 病（AOSD）患者中的相互作用。使用了 22 例 AOSD 患者和 22 名健康对照者（HC）的外周血单核细胞。分离的单核细胞首先用巨噬细胞集落刺激因子培养分化为 M0 巨噬细胞，然后用脂多糖/干扰素-γ或白细胞介素-4 分别分化为 M1 或 M2 巨噬细胞。使用定量 RT-PCR 和流式细胞术评估 HLA-E 和 NKG2A/CD94 的表达水平。与 HC 相比，AOSD 患者的 M0 和 M2 巨噬细胞中的 HLA-E 表达明显更高，且与血清 C 反应蛋白水平和红细胞沉降率呈正相关。AOSD 患者的 CD4+和 CD8+T 细胞中的 NKG2A/CD94 表达明显高于 HC，但 NK 细胞中的表达无明显差异。在 AOSD 患者中，CD4+T 细胞中的 NKG2A 表达与 M0、M1 和 M2 巨噬细胞中的 HLA-E 表达呈正相关。CD8+T 细胞中的 CD94 表达与 M1 和 M2 巨噬细胞中的 HLA-E 表达呈负相关。NK 细胞中的 NKG2A 和 CD94 表达与 M0、M1 和 M2 巨噬细胞中的 HLA-E 表达呈负相关。HC 中未观察到 HLA-E 与 NKG2A/CD94 表达之间的显著相关性。在 AOSD 的炎症状态下，可以观察到巨噬细胞中 HLA-E 的表达增加和 T 细胞中的 NKG2A/CD94 表达增加。表达 HLA-E 的巨噬细胞可能与 T 和 NK 细胞中 NKG2A/CD94 表达相关，其相关性不同。
