Lu Jianyun, Zhang Tong, Zhou Lu, Tong Xiaoliang, Gui Rong, Jiang Ling, Tang Zhen, Fu Yunfeng, Zhao Guosheng, Zeng Jinrong, Gao Lihua
Departments of Dermatology, The Third Xiangya Hospital, Central South University, Changsha, China.
Xiangya Medical College, Central South University, Changsha, China.
Regen Ther. 2024 Jun 7;26:213-218. doi: 10.1016/j.reth.2024.05.013. eCollection 2024 Jun.
Ozone can enhance the expression of some growth factors (GFs) in platelet rich plasma (PRP), recent study showed oxygen-rich PRP (ozonized PRP) have better therapeutic effects on bone and joint diseases. PRP injection has been widely used in the treatment of facial rejuvenation, but the efficacy of sufficient oxygen-rich PRP in facial rejuvenation has not been studied.
Firstly, we examined whether ozone treatment can increase the concentration of GFs of PRP in vitro. And then a variety of subjective and objective detection methods were used to evaluate the effect of sufficient(10-12 mL each time for the injection of face and neck) oxygen-rich (ozonized PRP) PRP injection in facial rejuvenation by follow-up for 6 months. At last, we investigated the satisfaction, side effects and pain score of the treatment through a questionnaire survey.
The concentration of main GFs in PRP treated with different dose of ozone in vitro was measured by ELISA. Clinical picture, the collagen thickness of dermis by reflectance confocal microscope(RCM), skin conditions (including spots, ultraviolet (UV) spots, brown spots, red area, pores, wrinkles, texture and porphyrin) by VISIA were collected before treatment and each month follow-up visit after treatment until 6-month follow-up period was finished. Patients' satisfaction, side effects and pain score were collected at the end of follow-up period.
PRP treated by high-dose ozone (57 μg/mL, ozone/PRP volume ratio:1/1) in vitro showed a significant increase in endothelial growth factor (EGF) and transforming growth factor-β (TGF-β) compared to baseline( < 0.05). Collagen thickness of forehead, cheek and neck improved significantly compare to the baseline until to the 6 months after treatment. Spots, UV spots, brown spots, red area and texture improved significantly compare to the baseline( < 0.05). All of participants reported improvement and have a median pain score of 4.19. No serious adverse events were observed.
Ozone treatment can increase the concentration of GFs such as EGF and TGF-β in PRP in vitro. Sufficient oxygen-rich PRP injection may be an effective and promising method to treat facial rejuvenation.
臭氧可增强富血小板血浆（PRP）中某些生长因子（GFs）的表达，近期研究表明富氧PRP（臭氧处理的PRP）对骨和关节疾病具有更好的治疗效果。PRP注射已广泛应用于面部年轻化治疗，但充足的富氧PRP在面部年轻化中的疗效尚未得到研究。
首先，我们研究了臭氧处理是否能在体外增加PRP中GFs的浓度。然后，采用多种主观和客观检测方法，通过6个月的随访来评估充足（每次面部和颈部注射10 - 12 mL）的富氧（臭氧处理的PRP）PRP注射在面部年轻化中的效果。最后，通过问卷调查来调查治疗的满意度、副作用和疼痛评分。
采用酶联免疫吸附测定（ELISA）法测定体外不同剂量臭氧处理的PRP中主要GFs的浓度。在治疗前及治疗后每月随访直至6个月随访期结束时，收集临床照片、用反射式共聚焦显微镜（RCM）测量真皮的胶原蛋白厚度、用VISIA检测皮肤状况（包括斑点、紫外线（UV）斑、褐色斑、红色区域、毛孔、皱纹、质地和卟啉）。在随访期结束时收集患者的满意度、副作用和疼痛评分。
体外经高剂量臭氧（57μg/mL，臭氧/PRP体积比为1/1）处理的PRP与基线相比，内皮生长因子（EGF）和转化生长因子-β（TGF-β）显著增加（P < 0.05）。与基线相比，前额、脸颊和颈部的胶原蛋白厚度在治疗后直至6个月时显著改善。与基线相比，斑点、UV斑、褐色斑、红色区域和质地显著改善（P < 0.05）。所有参与者均报告有改善，疼痛评分中位数为4.19。未观察到严重不良事件。
臭氧处理可在体外增加PRP中EGF和TGF-β等GFs的浓度。充足的富氧PRP注射可能是一种有效且有前景的面部年轻化治疗方法。
Zotero 插件
