Department of Biotechnology and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, Milano 20126, Italy.
Department of Biotechnology and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, Milano 20126, Italy.
Bioresour Technol. 2024 Aug;406:131063. doi: 10.1016/j.biortech.2024.131063. Epub 2024 Jul 2.
Responsible use of natural resources and waste reduction are key concepts in bioeconomy. This study demonstrates that agro-food derived-biomasses from the Italian food industry, such as crude glycerol and cheese whey permeate (CWP), can be combined in a high-density fed-batch culture to produce a recombinant β-galactosidase from Marinomonas sp. ef1 (M-βGal). In a small-scale process (1.5 L) using 250 mL of crude glycerol and 300 mL of lactose-rich CWP, approximately 2000 kU of recombinant M-βGal were successfully produced along with 30 g of galactose accumulated in the culture medium. The purified M-βGal exhibited high hydrolysis efficiency in lactose-rich matrices, with hydrolysis yields of 82 % in skimmed milk at 4 °C and 94 % in CWP at 50 °C, highlighting its biotechnological potential. This approach demonstrates the effective use of crude glycerol and CWP in sustainable and cost-effective high-density Escherichia coli cultures, potentially applicable to recombinant production of various proteins.
负责任地利用自然资源和减少废物是生物经济的关键概念。本研究表明，来自意大利食品工业的农业食品衍生生物量，如粗甘油和奶酪乳清渗透物（CWP），可以在高密度分批培养中组合使用，以生产来自 Marinomonas sp. ef1（M-βGal）的重组β-半乳糖苷酶。在使用 250 毫升粗甘油和 300 毫升富含乳糖的 CWP 的小规模工艺（1.5 L）中，成功生产了约 2000 kU 的重组 M-βGal 酶，同时在培养基中积累了 30 克半乳糖。纯化的 M-βGal 在富含乳糖的基质中表现出很高的水解效率，在 4°C 的脱脂乳中水解率为 82%，在 50°C 的 CWP 中水解率为 94%，突出了其生物技术潜力。这种方法展示了在可持续和具有成本效益的高密度大肠杆菌培养中有效利用粗甘油和 CWP 的潜力，可能适用于各种蛋白质的重组生产。
