Allison L A, Moyle M, Shales M, Ingles C J
Cell. 1985 Sep;42(2):599-610. doi: 10.1016/0092-8674(85)90117-5.
We have determined the nucleotide sequence of two yeast RNA polymerase genes, RPO21 and RPO31, which encode the largest subunits of RNA polymerases II and III, respectively. The RPO21 and RPO31 sequences are homologous to each other, to the sequence of the largest subunit of E. coli RNA polymerase, and to sequences in the putative DNA-binding domain of E. coli DNA polymerase I. RPO21 has an unusual heptapeptide sequence tandemly repeated 26 times at its C-terminus; this sequence is conserved in the RNA polymerase II of higher eukaryotes and may play an important role in polymerase II-mediated transcription. Since eukaryotic and prokaryotic RNA polymerases appear to have evolved from a common ancestral polymerase, other features of the transcription process may also be evolutionarily conserved.
我们已经确定了两个酵母RNA聚合酶基因RPO21和RPO31的核苷酸序列，它们分别编码RNA聚合酶II和III的最大亚基。RPO21和RPO31序列彼此同源，与大肠杆菌RNA聚合酶最大亚基的序列同源，也与大肠杆菌DNA聚合酶I推定的DNA结合结构域中的序列同源。RPO21在其C末端有一个不寻常的七肽序列串联重复26次；该序列在高等真核生物的RNA聚合酶II中保守，可能在聚合酶II介导的转录中起重要作用。由于真核和原核RNA聚合酶似乎是从共同的祖先聚合酶进化而来，转录过程的其他特征也可能在进化上保守。
